Cellular effects of peptide and protein hydroperoxides

Free Radical Biology & Medicine
Aldwin Suryo RahmantoMichael J Davies

Abstract

Hydroperoxides are major products of the reactions of radicals and singlet oxygen with amino acids, peptides, and proteins. These species can generate radicals in the presence of metal ions and oxidize thiols via nonradical reactions, but the effects of these materials on cells are poorly understood. In this study the exposure of murine macrophage-like cells to preformed peptide or protein hydroperoxides is shown to result in hydroperoxide consumption and cellular thiol oxidation; these effects precede loss of cell viability. N-acetyltryptophan methyl ester hydroperoxides, but not the decomposed species, decreased total cellular thiols and GSH, with the latter occurring more rapidly. Time-dependent inhibition of lysosomal cathepsins B and L was also observed, together with diminished caspase 3/7 activity. A number of other cytosolic thiol- and non-thiol-dependent enzymes were not affected significantly. Hydroperoxides formed on BSA did not deplete total thiols or GSH within cells, although such reactions are rapid in model systems. In contrast, selective inhibition of cathepsins B and L (but not cathepsin D or arylsulfatase) of the endosomal-lysosomal system was detected, consistent with localization within these compartments. ...Continue Reading

Citations

Feb 8, 2011·Journal of Clinical Biochemistry and Nutrition·Michael J Davies
Mar 4, 2014·Journal of Photochemistry and Photobiology. B, Biology·Joshua D WilliamsGeorg T Wondrak
Oct 22, 2011·Free Radical Biology & Medicine·Aldwin Suryo Rahmanto, Michael J Davies
Aug 17, 2010·Free Radical Biology & Medicine·Aldwin Suryo RahmantoMichael J Davies
Sep 16, 2010·The Biochemical Journal·Alexander V PeskinChristine C Winterbourn
Dec 24, 2013·Biochemical and Biophysical Research Communications·Vadim I BruskovSergey V Gudkov
May 14, 2011·Free Radical Biology & Medicine·Rodney P Guttmann, Sarbani Ghoshal
Aug 23, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·David I PattisonMichael J Davies
Feb 24, 2017·International Journal of Molecular Sciences·Fumiaki ItoKenji Hamaoka
Oct 16, 2010·Photochemistry and Photobiology·Sarah D LamoreGeorg T Wondrak
Mar 13, 2012·International Journal of Molecular Sciences·Cleva Villanueva, Robert D Kross
Oct 15, 2018·Journal of Cellular Physiology·Habib YaribeygiAmirhossein Sahebkar
Sep 25, 2019·Biomolecules·Thuluz Meza-MenchacaÁngel Trigos
Mar 18, 2020·International Journal of Molecular Sciences·Gilles LalmanachMylène Wartenberg
Mar 31, 2016·The Biochemical Journal·Michael J Davies
Jun 15, 2014·The Journal of Biological Chemistry·Radoslaw MichalskiBalaraman Kalyanaraman
Mar 10, 2020·Oxidative Medicine and Cellular Longevity·Jeremiah Oshiomame Unuofin, Sogolo Lucky Lebelo
Mar 10, 2021·Andrologia·Fernando LopesRosário Pinto-Leite
Sep 10, 2019·Chemical Reviews·Ari ZeidaRafael Radi

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