Cellular evaluation of diselenonicotinamide (DSNA) as a radioprotector against cell death and DNA damage

Metallomics : Integrated Biometal Science
M RaghuramanK Indira Priyadarsini

Abstract

Diselenonicotinamide (DSNA), a synthetic organoselenium compound, was evaluated for its radioprotective effect in cellular models. A clonogenic assay in Chinese Hamster Ovary (CHO) cells and an apoptosis assay in murine splenic lymphocytes indicated that pre-treatment with DSNA at a concentration of 25 μM significantly protected them from radiation-induced cell death. Upon irradiation (1-12 Gy), dose-response studies were carried out under similar treatment conditions, and its dose modification factor (DMF) was estimated to be 1.26. Furthermore, DSNA showed its radioprotective effect, even when administered after exposure to radiation. Mechanistic investigation revealed that DSNA increased the intracellular levels of GPx and GSH in irradiated cells. In line with this observation, the addition of a pharmacological inhibitor of GPx cycle, abrogated the activity of DSNA. The radioprotective effect of DSNA was also complemented by its ability to prevent radiation-induced DNA damage as monitored by micronucleus and γ-H2AX assays. Furthermore, treatment with DSNA did not show much change in the expressions of Nrf2 dependent genes (γ-GCL and HO-1), but the presence of a pharmacological inhibitor of Nrf2 abrogated the radioprotective a...Continue Reading

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Citations

Jun 14, 2019·Journal of Cellular Biochemistry·Bagher FarhoodAmirhossein Ahmadi
Jun 11, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Vimal K Jain
Feb 9, 2021·Free Radical Research·Beena G Singh, Amit Kunwar
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