Cellular events leading to chondrocyte death after cartilage impact injury

Arthritis and Rheumatism
D M GreenH H Birdsall

Abstract

We undertook this study to test our postulate that leukocytes extend the zone of injury in cartilage after acute mechanical trauma. Fresh cadaveric canine femoral condyles were subjected to 20-25-MPa impact injury. Condyle explants or dispersed chondrocytes were cultured with autologous blood mononuclear leukocytes (MNLs). Viability of chondrocytes at varying distances from the impact site was assessed by trypan blue exclusion. Mechanical injury caused a significant loss of viable chondrocytes over 7 days, even in cartilage >10 mm from the impact site. After biomechanical stress, death of cells within 10 mm of the impact could be largely prevented by addition of N(G)-monomethyl-L-arginine to inhibit nitric oxide (NO) generation. Chondrocytes within 10 mm of the impact were also susceptible to killing by living MNLs, but not by incubation with the supernatants of endotoxin-activated MNLs. Chondrocytes in this vulnerable zone expressed intercellular adhesion molecule 1 (ICAM-1) (CD54), facilitating attachment of MNLs that localized adjacent to the chondrocytes. Leukocytes killed dispersed chondrocytes harvested from the impact zone by generation of reactive oxygen species. Leukocyte-mediated killing could be blocked by desferoxam...Continue Reading

Associated Clinical Trials

Jun 25, 2015·Brett Crist, Brett Crist

References

Aug 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M L EntmanC W Smith
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W A HodgeR W Mann
Jul 17, 1999·The American Journal of Physiology·M G Simms, K R Walley
Oct 26, 1999·Journal of Biomechanical Engineering·P A TorzilliD L Helfet
Sep 15, 2000·Rheumatology·R J van't HofS H Ralston
Oct 14, 2000·Archives of Biochemistry and Biophysics·A M LoeningM W Lark
Feb 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G JinS Chien
Sep 20, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T AizawaL C Gerstenfeld
Nov 1, 2001·The Journal of Bone and Joint Surgery. American Volume·D D D'LimaC W Colwell
Feb 14, 2002·Osteoarthritis and Cartilage·D D D'LimaM K Lotz
Feb 13, 2002·Arthritis and Rheumatism·Marcello Del Carlo, Richard F Loeser
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tonia VincentJeremy Saklatvala
Sep 13, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L C GerstenfeldT A Einhorn
Aug 19, 2004·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·T MatsushitaC Hamanishi
Jun 7, 2005·Osteoarthritis and Cartilage·Y HenrotinT Aigner
Oct 4, 2006·The Journal of Bone and Joint Surgery. American Volume·D M GreenH H Birdsall

❮ Previous
Next ❯

Citations

Aug 26, 2010·Journal of Orthopaedic Trauma·David M BearConstance R Chu
Nov 16, 2007·Arthritis Research & Therapy·Miguel Otero, Mary B Goldring
Jul 31, 2010·International Journal of Clinical Rheumatology·Shawn P Grogan, Darryl D D'Lima
Aug 3, 2011·Orthopedic Reviews·Christoph ZiskovenRüdiger Krauspe
May 7, 2008·Current Rheumatology Reports·Marcello Del Carlo, Richard F Loeser
Jul 29, 2011·Canadian Journal of Physiology and Pharmacology·Christoph ZiskovenRüdiger Krauspe
Jan 15, 2013·Rheumatic Diseases Clinics of North America·David R WilsonJames D Johnston
Apr 11, 2012·Bone·Martin Lotz, Richard F Loeser
Dec 9, 2008·The Medical Clinics of North America·David R WilsonJames D Johnston
Aug 9, 2008·Rheumatic Diseases Clinics of North America·David R WilsonJames D Johnston
Jun 13, 2015·Musculoskeletal Surgery·A SpezialiG Cerulli
Sep 4, 2010·Osteoarthritis and Cartilage·L DingJ A Martin
Jan 16, 2014·Sports Health·Ayala Luria, Constance R Chu
Mar 16, 2017·The Journal of the American Academy of Orthopaedic Surgeons·Muhammad Farooq RaiFarshid Guilak
Jun 12, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yuya KawaraiSeiji Ohtori
Feb 23, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michelle L DelcoLisa A Fortier
May 29, 2008·Clinical Orthopaedics and Related Research·Hubert T KimAlexis C Dang
Jan 3, 2014·The Journal of the American Academy of Orthopaedic Surgeons·Mara L SchenkerSamir Mehta
Mar 8, 2017·Journal of Biomechanical Engineering·David J HeckelsmillerJessica E Goetz
Apr 12, 2020·Brazilian Journal of Physical Therapy·Fernando Augusto VasilceacStela Márcia Mattiello

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Camille A M Huser, M Elisabeth Davies
The Journal of Bone and Joint Surgery. British Volume
R A WhitesideP Mainil-Varlet
Cellular and Molecular Life Sciences : CMLS
T Aigner, L McKenna
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
C T ChenG Lust
© 2022 Meta ULC. All rights reserved