Cellular expression patterns of acetylcholinesterase activity during grasshopper development

Cell and Tissue Research
G BickerAnnely Haase

Abstract

We examined the expression of acetylcholinesterase (AChE) in the nervous system and epidermal body structures during embryonic and larval development of two grasshopper species: Locusta migratoria and Schistocerca americana. Histochemical labelling was blocked by the enzyme inhibitors eserine and BW284c51, but not by iso-OMPA, showing that the staining reflected true AChE activity. The majority of staining was localized on the cell surface but granular intracellular staining was also visible in many cell bodies. In both species, the cellular expression of AChE followed a similar but complex spatiotemporal staining pattern. Initially, mainly epidermal tissue structures were stained in the various body appendages (stages 25%-30%). Labelling subsequently appeared in outgrowing neurons of the central nervous system (CNS) and in the nerves innervating the limbs and dorsal body wall (stages 30%-40%). The latter staining originated in motoneurons of the ventral nerve cord. In a third phase (after 45%), the somata of certain identified mechanosensory neurons started to express AChE activity, presumably reflecting cholinergic differentiation. Staining was also found in repo-positive glial cells of the CNS, longitudinal glia of connectiv...Continue Reading

Citations

Jan 13, 2009·Journal of Insect Physiology·Maneesh KumarManchikatla Venkat Rajam
Jan 11, 2017·Journal of Neurochemistry·Ignaz Karl Wessler, Charles James Kirkpatrick
Jul 8, 2017·Development·Melissa A PickettNanette M Nascone-Yoder
Aug 21, 2018·Frontiers in Zoology·Hendrik LangelohMichael Stern
Aug 8, 2020·Apoptosis : an International Journal on Programmed Cell Death·Debbra Y KnorrRalf Heinrich
May 13, 2021·Scientific Reports·Gregor A Bergmann, Gerd Bicker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Related Papers

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Natalie A JenningsStephen A Watts
Journal of Cellular Biochemistry
Matthew D ThullberyKathleen M George
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Robert J Parker, Vanessa J Auld
Trends in Neurosciences
Carole Chotard, Iris Salecker
© 2022 Meta ULC. All rights reserved