Cellular functions of cholesterol probed with optical biosensors

Biochimica Et Biophysica Acta
Ye FangGuangshan Li

Abstract

Cholesterol is an essential constituent of cell membranes and the regulation of cholesterol concentration is critical for cell functions including signaling. In this paper, we applied resonant waveguide grating (RWG) biosensor to study the cellular functions of cholesterol through real time monitoring the dynamic mass redistribution (DMR) mediated by cholesterol depletion with methyl-beta-cyclodextrin (mbetaCD). In A431 cells, depletion of cholesterol by mbetaCD led to a DMR signature that was similar, but not identical to that induced by epidermal growth factor (EGF). To elucidate the cellular mechanisms of the DMR signal mediated by cholesterol depletion, a panel of modulators that specifically modulate the activities of various cellular targets were used to pretreat the cells. Results showed that the DMR signals triggered by cholesterol depletion are primarily linked to the transactivation of EGF receptor. Multiple signaling pathways including Ras/mitogenic activated protein (MAP) kinase, protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K) acted synergically in the cell response, whereas the activation of protein kinase A (PKA) pathway was found to antagonize the cell response.

References

Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M C MaaS J Parsons
Oct 1, 1994·Molecular and Cellular Biology·S HäfnerW Kolch
Apr 26, 1996·The Journal of Biological Chemistry·P LiuR G Anderson
Aug 8, 1998·The Journal of Biological Chemistry·T Furuchi, R G Anderson
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A SubtilT E McGraw
Oct 19, 1999·Molecular and Cellular Biology·E J SmartM P Lisanti
Mar 14, 2000·Current Opinion in Cell Biology·J P Incardona, S Eaton
Apr 12, 2000·Biochemistry·M P HaynesG H Rothblat
Nov 1, 2000·Cell·J Schlessinger
Dec 2, 2000·Science·K Simons, E Ikonen
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Aug 30, 2001·Cell·F GalbiatiM P Lisanti
Oct 30, 2001·Experimental Biology and Medicine·F SchroederA B Kier
Feb 16, 2002·European Journal of Biochemistry·Laura D Zajchowski, Stephen M Robbins
May 9, 2002·Molecular and Cellular Biology·Nicolas DumazRichard Marais
Jul 18, 2002·Nature Reviews. Drug Discovery·Matthew A Cooper
May 30, 2003·The EMBO Journal·Ping-Yuan WangRichard G W Anderson
Aug 19, 2003·Nature Reviews. Molecular Cell Biology·Reinhard Wetzker, Frank-D Böhmer
Oct 19, 2004·Oncogene·Deirdre K Luttrell, Louis M Luttrell
Mar 5, 2005·Science·Ping-Yuan WangRichard G W Anderson
Jul 26, 2005·FEBS Letters·Ye FangGuangshan Li

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Citations

Nov 23, 2006·Assay and Drug Development Technologies·Ye Fang
Aug 29, 2013·Journal of Microbiological Methods·Guangshan LiAnping Liu
May 12, 2009·Seminars in Cell & Developmental Biology·M N Velasco-Garcia
Sep 20, 2016·IEEE Sensors Journal·B T CunninghamC Race
Jun 13, 2006·Biophysical Journal·Ye FangJitendra Balakrishnan
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka

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