PMID: 3754881Jul 1, 1986Paper

Cellular heterogeneity in normal human urothelium: an analysis of optical properties and lectin binding

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
G K WardW J Mackillop

Abstract

Flow cytometry was used to study the optical properties of normal urothelial cells in suspension. Narrow-angle light scatter, which is a function of cell size, defined one major and one minor cell population, and 90 degrees light scatter, a function of intracellular structure, showed three distinct cell populations. These properties were displayed as a 2-dimensional dot plot or "fingerprint" which proved to be characteristic and reproducible from one specimen of urothelium to the next. Cell sorting on the basis of these two parameters demonstrated that the small cells of the basal layer occupy the low narrow angle, low 90 degrees light-scatter region; the giant cells of the superficial layer lie in the high narrow angle, high 90 degrees scatter region; and the pyramidal cells of the intermediate layer lie in an intermediate zone. Studies of tissue sections using the galactose-specific, FITC-conjugated Maclura Pomifera lectin (MPA) demonstrated preferential binding to the superficial layers of intact urothelium. In order to quantify the apparent differences in lectin binding between the superficial and basal layers, urothelial cell suspensions were labeled with FITC-conjugated MPA and studied by flow cytometry. The resolution ob...Continue Reading

Citations

Jun 1, 1987·The Histochemical Journal·G K WardW J Mackillop
Jul 27, 2010·Current Opinion in Urology·Keith Syson ChanIrving Weissman
Jan 6, 2016·International Journal of Molecular Sciences·Tomokazu OhishiToshiro Migita
May 17, 2007·International Journal of Experimental Pathology·Ahmad KeshtkarPat Lawford
Jun 1, 1990·Experimental Cell Research·G K WardG B Price
Sep 25, 2004·Journal of Cellular Physiology·Amber E HudsonJames A Bassuk

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