PMID: 4893888Aug 1, 1969Paper

Cellular immunity in vitro. Clonal proliferation of antigen-stimulated lymphocytes

The Journal of Experimental Medicine
W H MarshallH S Lawrence

Abstract

When sensitive lymphocytes are cultured with the appropriate antigen, lymphoblasts appear after 24-48 hr of incubation and the number of these increases steadily from the 2nd to the 6th or 7th day. Our problem was to discover, at a cellular level, how this increase takes place; whether it is a massive response of many cells, stepwise recruitment of cells into the lymphoblast class, or simply repeated division of a few cells to form clones. In these experiments lymphocytes were incubated with antigen in culture tubes for 2-4 days and then a few cells, usually less than 200, were transferred to special microchambers for further culture. In these microchambers the cells could be viewed continually with a microscope and their fate recorded over the next 3-5 days by time-lapse cinemicrography. Examination of the film produced in this way showed that lymphoblasts divided and redivided to produce clones of 64 cells or more. It was possible to measure generation times from the film for 301 cells; the majority were between 8 and 13 hr but the range was 7.5-38.0 hr. There was no clear difference between generation times of human lymphocytes stimulated with tuberculin, streptokinase-streptodrnase, extract of the American pokeweed, or in t...Continue Reading

References

Mar 23, 1963·Lancet·G PEARMAINP H FITZGERALD

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Citations

Jan 1, 1984·Zeitschrift Für Parasitenkunde·M BaileyE J Soulsby
Sep 1, 1973·Cellular Immunology·J P RevillardM Robert
Jul 16, 2003·The Journal of Infection·Angela C KimRobert S Holzman
Aug 20, 1970·The New England Journal of Medicine·H S Lawrence
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J C RogersC R Valeri
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J L BernheimR Dorian
Apr 1, 1974·The Journal of Cell Biology·N A Berger, A M Skinner
Aug 1, 1970·The Journal of Experimental Medicine·A L LuzzatiA O Carbonara
May 1, 1974·The Journal of Experimental Medicine·H P LohrmannR G Graw
Oct 1, 1975·The Journal of Experimental Medicine·H Polet, H Spieker-Polet
May 1, 1976·The Journal of Experimental Medicine·J C Rogers
Jun 1, 1976·The Journal of Experimental Medicine·M A Skinner, J Marbrook
Feb 13, 1970·Annals of the New York Academy of Sciences·H S Lawrence, F T Valentine
Jan 1, 1980·Cell and Tissue Kinetics·L R HanrahanA Rosenberg
Sep 1, 1972·Cellular Immunology·E Hawrylko, G B Mackaness
Dec 5, 1973·Nature: New Biology·H Ginsburg, J Nicolet
Jan 14, 2016·Transplantation·Susan DeWolfMegan Sykes
May 15, 1981·Klinische Wochenschrift·M WinkelmeyerF W Eigler
Jan 1, 1970·Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology·B Lund, O M Jensen

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