Cellular impact of combinations of endosulfan, atrazine, and chlorpyrifos on human primary hepatocytes and HepaRG cells after short and chronic exposures

Cell Biology and Toxicology
Ahmad NawazR Rahmani

Abstract

Chronic exposure to low doses of pesticides present in the environment is increasingly suspected to cause major health issues to humans. Toxicological evaluations become more complex when the exposure concerns chemical combinations. Atrazine, chlorpyrifos, and endosulfan are pesticides used worldwide in agriculture and are therefore currently found at residual levels in food and the environment, even in countries in which they are now banned. Our study aimed to use Real-Time Cell Impedance Analyzer to investigate changes in phenotypical status of primary human hepatocytes and differentiated HepaRG cells induced by short and chronic exposures to these three chemicals. In contrast to the traditionally used endpoint cytotoxicity test, this technology allows kinetic measurements in real-time throughout the entire experiment. Our data show significantly higher cytotoxic effects of mixtures as compared to individual pesticides and a greater susceptibility of human hepatocytes as compared to HepaRG to short-term exposure (24 h). Repeated exposure over 2 weeks to endosulfan and endosulfan-containing mixture induced HepaRG cell death in a time- and dose-dependent manner. Of the typical genes involved in metabolism and cell-response to x...Continue Reading

References

Apr 1, 1996·Environmental Health Perspectives·V F GarryJ Griffith
Nov 1, 1995·Environmental Health Perspectives·A Blair, S H Zahm
May 2, 2000·Journal of Biochemical and Molecular Toxicology·E LeCluyseA Parkinson
Jul 27, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·P C DasR L Cooper
May 9, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J SugataniT Sueyoshi
Sep 7, 2002·Mental Retardation and Developmental Disabilities Research Reviews·Pauline MendolaDeborah Rice
Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Philippe GriponChristiane Guguen-Guillouzo
Jan 1, 2003·Archives of Environmental Health·Jack D ThrasherAlan Broughton
Mar 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wen XieRonald M Evans
Apr 29, 2003·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Frederick N Bebe, Myna Panemangalore
Jul 23, 2003·Current Drug Metabolism·M J Gómez-LechónR Jover
Jan 8, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Matthew G SoarsSteven A Wrighton
Sep 11, 2004·Assay and Drug Development Technologies·Kelli SollyWei Zheng
Nov 19, 2004·Biochemical Pharmacology·Géraldine LemaireRoger Rahmani
Oct 20, 2005·Journal of Biomolecular Screening·Josephine M AtienzaYama Abassi
Jan 18, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Donald D Heistad
Mar 21, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Nobumitsu HaniokaShizuo Narimatsu
Jul 14, 2006·The Biochemical Journal·Laura RomeroGordon Kirby
Oct 5, 2006·Journal of Neural Transmission. Supplementum·A R ChadeC M Tanner
Jan 16, 2007·Drug Metabolism and Pharmacokinetics·Kotoko NakataToshihisa Ishikawa
Jan 24, 2007·Chemico-biological Interactions·André GuillouzoChristiane Guguen-Guillouzo
Jul 5, 2007·Pediatric Blood & Cancer·Katherine M LafiuraYubin Ge
Dec 19, 2007·International Journal of Occupational and Environmental Health·Frank Ackerman
Apr 17, 2008·Biotechnology Journal·Biao XiYama A Abassi
Dec 17, 2008·Toxicology and Applied Pharmacology·Carine B LambertAndre Guillouzo
Feb 27, 2009·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Marilyn H Silva, Derek Gammon
Jan 1, 2008·Drug Metabolism and Drug Interactions·Parikshit C DasErnest Hodgson
Aug 26, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jie Gao, Wen Xie
Dec 15, 2010·Neurotoxicology·Oscar U ScreminMiguel A Lucero
Jun 3, 2011·European Journal of Epidemiology·Karin WirdefeldtJack Mandel
Sep 13, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I M Medina-DíazG Elizondo
Feb 9, 2012·Toxicology·Khaled AbassJukka Hakkola
Apr 21, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Patrick RouimiRoger Rahmani
Jul 26, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Junko SugataniMasao Miwa

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Citations

Aug 5, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ludovic PeyreRoger Rahmani
Apr 2, 2015·Toxicology Letters·S TengA R R Pery
Jun 19, 2016·Toxicology and Applied Pharmacology·S TengA R R Pery
Jun 18, 2016·Chemico-biological Interactions·V RizzatiL Gamet-Payrastre
May 6, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ludovic PeyreRoger Rahmani
Sep 14, 2016·Biochemical and Biophysical Research Communications·Deepika AroraYogeshwer Shukla
Nov 5, 2014·Environmental Science & Technology·Genevieve M ForsterElizabeth P Ryan
Jul 17, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Georges de SousaRoger Rahmani
Dec 14, 2019·Environmental Science and Pollution Research International·Ana Paola Balderrama-CarmonaEdgar Felipe Morán-Palacio
Jul 14, 2020·Environmental Science and Pollution Research International·Monika JainMika Sillanpää
Apr 6, 2020·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Benjamin KoppMarc Audebert

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