Cellular internalization of lactoferrin in intestinal epithelial cells

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Kinya AshidaBo Lönnerdal

Abstract

We studied the cellular internalization of lactoferrin (Lf) in an intestinal epithelial cell line, Caco-2, to investigate the mechanism of biological actions of ingested Lf. RT-PCR and Western blotting analyses revealed that differentiated Caco-2 cells express LfR mRNA and its protein with a 34 kD molecular weight under reducing conditions. Biotin-labeled Lf showed specific binding to the cellular membrane of differentiated Caco-2 cells with a dissociation constant (Kd) of 0.16 microM. The cellular internalization of Lf was studied in differentiated Caco-2 cells grown as monolayers on Transwell inserts, and compared to that of human transferrin (Tf). After labeling with fluorescent dye, either Lf or Tf was added to Caco-2 cells from the apical side or the basolateral one. Laser scanning confocal microscopy showed that labeled Lf was internalized only from the apical side and localized to the nuclei. On the other hand, labeled Tf was internalized from the basolateral side, not from the apical side, and localized in the cytoplasm. The internalization of labeled Lf was inhibited by excess of unlabeled Lf, but not of Tf. The internalization of labeled Lf, but not of labeled Tf, was also suppressed by heparin. This indicates that a ...Continue Reading

Citations

Oct 19, 2006·Journal of Biomedical Science·Rong-qin HuangChen Jiang
Apr 22, 2009·Molecular BioSystems·Valerie HowerSuzy V Torti
Jun 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert G ThorneCharles Nicholson
Jun 27, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Colleen S Curran, Paul J Bertics
Mar 26, 2009·Current Opinion in Clinical Nutrition and Metabolic Care·Bo Lönnerdal
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Francesca BerluttiPiera Valenti
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Veronica LopezBo Lönnerdal
Feb 2, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Pooja RawatManoj Raje
Feb 16, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yalin LiaoBo Lönnerdal
Mar 3, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Paula FlorianAnca Roseanu
May 12, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jeremy H Brock
Sep 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yoshihiko HirotaniKazuhiko Tanaka
Aug 2, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Anne BlaisDaniel Tomé
Aug 18, 2010·Cancer Metastasis Reviews·Elvira Gonzalez de Mejia, Vermont P Dia
Mar 2, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Patrizia PudduSandra Gessani
Jul 8, 2010·Journal of Gastroenterology·Sanne Mie NielsenE Michael Danielsen
Jul 14, 2010·Biotechnology Advances·Celia ConesaLourdes Sánchez
Nov 30, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yuki ShigaAtsushi Sato
Mar 24, 2009·Veterinary Immunology and Immunopathology·Morgane A Henry, Maria N Alexis
Mar 31, 2006·International Journal of Cancer. Journal International Du Cancer·Liv Tone EliassenØystein Rekdal
May 1, 2010·Plant Biotechnology Journal·Martin E BrandsmaShengwu Ma
Jan 24, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M J AppelF de Loos
Apr 23, 2011·Veterinary Microbiology·M Atef YektaD Vanrompay
Oct 20, 2011·The International Journal of Biochemistry & Cell Biology·Rulan Jiang, Bo Lönnerdal
Jan 18, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Rosa SessaPiera Valenti
Jan 25, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Delia MandracchiaGiuseppe Tripodo
Jul 11, 2007·Veterinary Research·Delphine Sylvie Anne BeeckmanDaisy C G Vanrompay
Sep 16, 2017·International Journal of Molecular Sciences·Luigi RosaPiera Valenti
Mar 9, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Luigi RosaPiera Valenti
Feb 22, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rulan Jiang, Bo Lönnerdal
Feb 18, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Maria Carmela Bonaccorsi di PattiGiovanni Musci
Apr 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Stefania LepantoAntimo Cutone
Jul 16, 2020·International Journal of Molecular Sciences·Elena CampioneLuca Bianchi
Mar 17, 2018·Frontiers in Immunology·Piera ValentiPaola Mastromarino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.