Cellular lead toxicity and metabolism in primary and clonal osteoblastic bone cells

Toxicology and Applied Pharmacology
G J LongJ G Pounds

Abstract

A knowledge of bone lead metabolism is critical for understanding the toxicological importance of bone lead, as a toxicant both to bone cells and to soft tissues of the body, as lead is mobilized from large reservoirs in hard tissues. To further understand the processes that mediate metabolism of lead in bone, it is necessary to determine lead metabolism at the cellular level. Experiments were conducted to determine the intracellular steady-state 210Pb kinetics in cultures of primary and clonal osteoblastic bone cells. Osteoblastic bone cells obtained by sequential collagenase digestion of mouse calvaria or rat osteosarcoma (ROS 17/2.8) cells were labeled with 210Pb as 5 microM lead acetate for 20 hr, and kinetic parameters were determined by measuring the efflux of 210Pb from the cells over a 210-min period. The intracellular metabolism of 210Pb was characterized by three kinetic pools of 210Pb in both cell types. Although the values of these parameters differed between the primary osteoblastic cells and ROS cells, the profile of 210Pb was remarkably similar in both cell types. Both types exhibited one large, slowly exchanging pool (S3), indicative of mitochondrial lead. These data show that primary osteoblastic bone cells and...Continue Reading

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Citations

Mar 5, 2008·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M E RuppelD B Burr
Oct 1, 1993·Clinical Biochemistry·G Lockitch
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Sep 28, 2010·Journal of Cellular Biochemistry·Su Hee HanSung-Jin Kim
Jun 13, 2006·Journal of Occupational and Environmental Medicine·Vijayalakshmi PotulaWendy Kaye
Jun 1, 2007·Birth Defects Research. Part C, Embryo Today : Reviews·Jonathan D HolzJ Edward Puzas
Jan 25, 2018·Military Medicine·Walter N NickelBenjamin K Potter
Aug 29, 2013·Clinical Orthopaedics and Related Research·John T RiehlKenneth J Koval
Dec 30, 2011·Menopause : the Journal of the North American Menopause Society·Geum Joon ChoTak Kim
Nov 1, 1990·Toxicology and Applied Pharmacology·G J LongJ G Pounds

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