Cellular localization and phosphorylation of Hrb1p is independent of Sky1p

Biochimica Et Biophysica Acta
Ziv PoratChaim Kahana

Abstract

Protein phosphorylation plays a major role in regulating cellular functions. We have previously demonstrated that Sky1p, the SR protein kinase of the budding yeast Saccharomyces cerevisiae, is a regulator of polyamine transport and ion homeostasis. Since its kinase activity was demonstrated essential for fulfilling these roles, we assumed that Sky1p function via substrates phosphorylation. Using an in vitro phosphorylation assay, we have identified Hrb1p as a putative Sky1p substrate. However, phosphorylation analysis in WT and sky1Delta cells and localization studies disproved Hrb1p as a true Sky1p substrate, although a segment of the RS domain is required for determining its subcellular localization. Furthermore, we demonstrate that Hrb1p and additional putative Sky1p substrates, identified by computational approach, are not involved in mediating the spermine tolerant phenotype of sky1Delta cells.

References

Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R PillaiJ Colicelli
Sep 14, 1994·Biochimica Et Biophysica Acta·T MaruyamaK Igarashi
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·C W SiebelX D Fu
Dec 28, 1999·Analytical Biochemistry·K Melcher
Feb 15, 2001·Nature Structural Biology·B NolenG Ghosh
Jun 8, 2002·Molecular and Cellular Biology·Mehdi KabaniJeffrey L Brodsky
Dec 17, 2003·The Journal of Biological Chemistry·Sabine Häcker, Heike Krebber
Jun 24, 2004·Progress in Nucleic Acid Research and Molecular Biology·Cyril F BourgeoisJames Stévenin
Mar 8, 2005·Molecular Cell·Yingqun Huang, Joan A Steitz
Apr 28, 2005·The Journal of Biological Chemistry·Mustapha AouidaDindial Ramotar

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Citations

Mar 26, 2013·Chromosoma·Zhihong Zhou, Xiang-Dong Fu
Jul 17, 2014·International Journal of Molecular Sciences·Silvia Rodríguez-LombarderoM Esperanza Cerdán

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