PMID: 11337939May 8, 2001Paper

Cellular localization and regional distribution of CYP2D6 mRNA and protein expression in human brain

Pharmacogenetics
I SiegleM Eichelbaum

Abstract

The cytochrome P4502D6 (CYP2D6) is involved in the biotransformation of many drugs which predominantly act in the central nervous system (CNS), including opioids, various psychotrophic drugs and neurotoxins. Until now, however, only controversial information is available regarding the presence of CYP2D6 in CNS. In this study, the regional and cellular expression of CYP2D6 transcripts and proteins in postmortem brain tissues of three individuals was analysed. A combination of in-situ hybridization coupled with immunohistochemistry on adjacent sections allowed simultaneous detection of CYP2D6 mRNA and protein. However, discrepancies existed in the results such that the mRNA was more widely distributed in the brain areas analysed compared to the protein. Neuronal cells, as well as glial cells, showed labelling for mRNA in brain regions such as the neocortex, caudate nucleus, putamen, globus pallidus, hippocampus, hypothalamus, thalamus, substantia nigra and cerebellum. In contrast, CYP2D6 protein was primarily localized in large principal neurons such as pyramidal cells of the cortex, pyramidal cells of the hippocampus, and Purkinje cells of the cerebellum. In glial cells, CYP2D6 protein was absent. These results provide clear evi...Continue Reading

References

Jan 1, 1990·General Pharmacology·A V Juorio, I A Paterson
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·U M ZangerU A Meyer
Jun 1, 1981·American Journal of Clinical Pathology·S M HsuH Fanger
Mar 1, 1995·Clinical Pharmacology and Therapeutics·J A AgúndezF Coria
Oct 1, 1993·Archives of Neurology·P Seeman
Mar 1, 1993·Archives of Biochemistry and Biophysics·S BhamreV Ravindranath
Feb 1, 1996·Acta Psychiatrica Scandinavica·U A MeyerA Delini-Stula
Mar 4, 1996·The Journal of Comparative Neurology·P J NorrisP C Emson
Jan 1, 1997·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D E GilhamC R Wolf
Mar 21, 1997·Biochemical Pharmacology·Y AmetJ F Ménez
Apr 1, 1997·Pharmacogenetics·C MartínezJ Benítez
Feb 5, 1998·Biochemical Society Transactions·M C McFadyenG I Murray
May 23, 1998·Biochimica Et Biophysica Acta·T HiroiY Funae
May 14, 1998·Neuroscience and Biobehavioral Reviews·G B BakerR T Coutts
Jun 19, 1998·Chemico-biological Interactions·P WolkensteinP Beaune
Sep 10, 1998·Biochemical and Biophysical Research Communications·T HiroiY Funae
Nov 17, 1998·International Clinical Psychopharmacology·K Brøsen
Mar 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H YangN Chaudhari
Mar 20, 1999·Brain Research·A G RiedlC D Marsden
Feb 16, 2000·Pharmacology & Therapeutics·C Hiemke, S Härtter
Mar 1, 2000·Brain Research·P VoirolP Baumann
Apr 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I SiegleM Kömhoff

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Citations

Jul 30, 2008·Amino Acids·M SamuelssonC Nordin
Sep 13, 2003·European Journal of Pharmacology·Philip HeiserHelmut Vedder
Apr 7, 2010·Molecular Psychiatry·J KirchheinerR Viviani
Feb 28, 2007·The Pharmacogenomics Journal·A LlerenaA De la Rubia
Apr 6, 2004·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·O SanttG Lucotte
Oct 2, 2002·Journal of Neurochemistry·Sharon MiksysRachel F Tyndale
Jun 30, 2009·The Journal of Biological Chemistry·Nadja GrobeF Peter Guengerich
Jan 26, 2007·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·R VilarM C Lechner
Aug 14, 2008·The Journal of Pharmacology and Experimental Therapeutics·Natasha T SniderPaul F Hollenberg
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·Frank J Gonzalez, Ai-Ming Yu
Apr 7, 2012·International Journal of Alzheimer's Disease·Ramón CacabelosMasatoshi Takeda
Dec 22, 2005·Anesthesia and Analgesia·Thomas P EnggaardSøren H Sindrup
Aug 11, 2004·Clinical Pharmacokinetics·Guillermo GervasiniJulio Benitez
Sep 19, 2009·Pharmacogenomics·Fabiana B KohlrauschMara H Hutz
Jan 1, 2014·European Journal of Pharmacology·Xiaoshuang WangJiang Yue
Mar 21, 2002·Expert Review of Molecular Diagnostics·R W KerwinM J Arranz
Sep 11, 2008·Pharmacogenomics·Parvaz Madadi, Gideon Koren
Apr 21, 2009·Pharmacogenomics·Monique J BijlBruno H Ch Stricker
Sep 1, 2008·Expert Review of Clinical Pharmacology·Sunao KanekoHiroto Iwasa
Jun 12, 2009·Drug Metabolism Reviews·Shu-Feng ZhouBalram Chowbay
Mar 6, 2010·Expert Opinion on Drug Metabolism & Toxicology·Ralf Peter Meyer, Marcel Gehlhaus
Mar 16, 2013·Expert Opinion on Drug Metabolism & Toxicology·Romina KlimasGerd Mikus
Jul 9, 2004·Drug Metabolism Reviews·Ai-Ming YuFrank J Gonzalez
Jul 9, 2004·Drug Metabolism Reviews·Sharon Miksys, Rachel F Tyndale
Oct 7, 2011·Trends in Pharmacological Sciences·Charmaine S Ferguson, Rachel F Tyndale
Sep 14, 2010·Molecular Genetics and Metabolism·T WassenbergM M Verbeek
Oct 13, 2009·European Journal of Pharmacology·Ewa BromekWładysława A Daniel
May 21, 2004·British Journal of Clinical Pharmacology·S H GanE T Larmie
Mar 14, 2009·Basic & Clinical Pharmacology & Toxicology·Stine T ZwislerSoeren H Sindrup
Sep 17, 2013·British Journal of Clinical Pharmacology·Eva M Peñas-Lledó, Adrián Llerena

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