PMID: 8955509Nov 1, 1996Paper

Cellular localization of antiviral polyoxometalates in J774 macrophages

Antiviral Research
L NiF D Boudinot

Abstract

The cellular localization of the polyoxometalates, K12H2[P2W12O48].24H20 (JM 1591), K10[P2W18-Zn4(H2O)2O68].20H2O (JM 1596), and [Me3NH]8[Si2W18Nb6O77] (JM 2820) were examined in cultured J774 cells by inhibition of cellular uptake of acetylated low-density lipoprotein (LDL) and by electron microscopy. All three polyoxometalates inhibited the cellular uptake of acetylated LDL, suggesting that the polyoxometalates block the association of acetylated LDL with cellular scavenger receptors. Fluorescence microscopy showed increased numbers of vacuoles in the presence of polyoxometalates, suggesting their uptake by cells. Using scanning electron microscopy (SEM), no significant cell surface morphological differences were observed between treated and non-treated J774 cells, suggesting that the compounds are not toxic to J774 cells up to a concentration of 200 micrograms/ml. Transmission electron microscopy (TEM) revealed large amounts of high electron dense granules were observed in the ramifying system of tubular cavities and vacuoles. TEM-energy dispersive spectroscopy (EDS) X-ray microanalysis was unable to differentiate the dense particles, most likely because the amount of tungsten in the cells was below the limit of detection. X...Continue Reading

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Citations

May 8, 2013·Journal of Photochemistry and Photobiology. B, Biology·S DianatP Habibi
Sep 8, 2015·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Ştefana BâliciHorea Matei
Oct 10, 2012·Carbohydrate Polymers·Georg GeisbergerGreta R Patzke
Jun 1, 2014·ACS Applied Materials & Interfaces·Juan WangZhenhui Kang
Sep 29, 2011·Small·Georg GeisbergerGreta R Patzke
Jun 13, 2018·Angewandte Chemie·Aleksandar BijelicAnnette Rompel
Feb 16, 2011·Chemistry : a European Journal·Georg GeisbergerGreta R Patzke
Jan 23, 2018·Chemical Communications : Chem Comm·Aleksandar BijelicAnnette Rompel
May 23, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Georg GeisbergerGreta R Patzke
Dec 11, 2013·Inorganic Chemistry·Il YoonYoung Key Shim
Jan 25, 2011·Bioorganic & Medicinal Chemistry Letters·Andreas FlütschGreta R Patzke

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