PMID: 9524198May 9, 1998Paper

Cellular localization of calmodulin-dependent protein kinases I and II to A-cells and D-cells of the endocrine pancreas

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
L M MatovcikF S Gorelick

Abstract

Ca2+/calmodulin-dependent protein kinases I and II, initially identified in brain on the basis of their ability to phosphorylate synapsin I, have been implicated in the regulation of Ca2+-dependent synaptic neurosecretion. Specific recombinant and synthetic peptide antibodies were used to examine the distribution of CaM kinases I and II in the rat pancreas and other tissues. The CaM kinase I antibodies detected a doublet of cytosolic proteins of approximately 38 and approximately 42 kD by immunoblot. CaM kinase I was observed in glucagon-containing A-cells at the periphery of the islet of Langerhans but had little or no overlap with pancreatic polypeptide or somatostatin cells. In contrast, CaM kinase II was localized to somatostatin-containing D-cells. CaM kinase I co-localized with glucagon secretory granules. CaM kinase II was not associated with the somatostatin granule but rather was enriched in areas of the cells that contained relatively little somatostatin. Because glucagon secretion is Ca2+-dependent, it is attractive to speculate that CaM kinase I may play a regulatory role in glucagon secretion. Glucagon and somatostatin cells both utilize intracellular Ca2+ for signaling. Therefore, specific CaM kinases may act as e...Continue Reading

References

Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P K DashE R Kandel
Aug 1, 1991·The Journal of Clinical Investigation·C R MarinoJ A Cohn
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A J CzernikP Greengard
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R LinM G Rosenfeld
May 1, 1983·Analytical Biochemistry·J M Gershoni, G E Palade
Jan 1, 1995·Annual Review of Physiology·A P Braun, H Schulman
Oct 20, 1994·Biochimica Et Biophysica Acta·F S ChoT E Weaver
Nov 30, 1994·Biochemical and Biophysical Research Communications·H P BodeJ A Williams
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C LeeA M Edelman
Feb 1, 1993·The Biochemical Journal·S J HughesS J Ashcroft
Jan 1, 1996·Advances in Pharmacology·M R PicciottoA C Nairn
Mar 1, 1997·Biochimica Et Biophysica Acta·R M Tombes, G W Krystal

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Citations

Jan 29, 1999·Physiological Reviews·D C Gadsby, A C Nairn

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