Cellular localization of facilitated glucose transporter 1 (GLUT-1) in the cochlear stria vascularis: its possible contribution to the transcellular glucose pathway

Cell and Tissue Research
M AndoShunji Takeuchi

Abstract

Immunoreactivity for the facilitated glucose transporter 1 (GLUT-1) has been found in the cochlear stria vascularis, but whether the strial marginal cells are immunopositive for GLUT-1 remains uncertain. To determine the cellular localization of GLUT-1 and to clarify the glucose pathway in the stria vascularis of rats and guinea pigs, immunohistochemistry was performed on sections, dissociated cells, and whole-tissue preparations. Immunoreactivity for GLUT-1 in sections was observed in the basal side of the strial tissue and in capillaries in both rats and guinea pigs. However, the distribution of the positive signals within the guinea pig strial tissue was more diffuse than that in rats. Immunostaining of dissociated guinea pig strial cells revealed GLUT-1 in the basal cells and capillary endothelial cells, but not in the marginal cells. These results indicated that GLUT-1 was not expressed in the marginal cells, and that another isoform of GLUT was probably expressed in these cells. Three-dimensional observation of whole-tissue preparations demonstrated that cytoplasmic prolongations from basal cells extended upward to the apical surface of the stria vascularis from rats and guinea pigs, and that the marginal cells were surro...Continue Reading

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Citations

Jan 30, 2016·Acta Oto-laryngologica·Mattia Carraro, Robert V Harrison
Apr 2, 2014·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Olubunmi Victoria AkinpeluSam J Daniel
Jun 1, 2018·Neural Plasticity·Xiao-Ting ChengNing Yu
Oct 12, 2017·Frontiers in Molecular Neuroscience·Mitsuo P SatoHiroshi Hibino
Oct 25, 2017·Frontiers in Cellular Neuroscience·Meiyan JiangPeter S Steyger
May 23, 2019·Audiology & Neuro-otology·Nathan HuerzelerDaniel Bodmer

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