PMID: 40415Jul 1, 1979

Cellular localization of Saint Louis encephalitis virus replication

Acta Virologica
I A BrawnerD W Trent


Replication of Saint Louis encephalitis (SLE) virus was inhibited when PS cells were treated with actinomycin D, daunomycin or cordycepin during the first 9 hr after infection. Autoradiography of SLE virus-infected pulse labelled cells demonstrated that viral RNA synthesis is localized within the nuclear area. Nuclei purified from cells after 12 hr of infection contained heterogeneous 20 S to 26 S viral RNA but no SLE virus genome sized 43 S RNA. Later during infection, nuclei isolated from infected cells contained large amounts of 43 S and 20 S to 26 S RNAs. The 43 S viral RNA present in cells late in infection could not be removed with 1% Tween 80: Nonidet P 40. Purified nuclei isolated from cells early in infection supported the synthesis of 43 S virion RNA in the absence of cytoplasmic factors. The cytoplasmic membrane fraction prepared from cells early in infection contained heterogeneous 10 S to 26 S RNA species; later during infection these membranes contained viral 43 S, 26 S to 30 S and 4 S RNA. These results suggest that the nucleus is an important site of early viral synthesis.

Related Concepts

Tissue Membrane
Cell Nucleus
Encephalitis Viruses
Tween 80
Encephalitis Virus, St. Louis

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.