Cellular localization of THIK-1 (K(2P)13.1) and THIK-2 (K(2P)12.1) K channels in the mammalian kidney

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Franziska TheiligChristian Derst

Abstract

K(+)-channels fulfill several important functions in the mammalian kidney such as volume regulation, recirculation and secretion of K(+) ions, and maintaining the resting potential. In this study we used immunocytochemical methods, in situ hybridization, and nephron segment-specific RT-PCR to obtain a detailed picture of the cellular localization of two tandem pore domain potassium (K(2P)) channels, THIK-1 (K(2P)13.1, KCNK13) and THIK-2 (K(2P)12.1, KCNK12). Monospecific antibodies against C-terminal domains of rat THIK-1 and THIK-2 proteins (GST-fusion proteins) were raised in rabbits, freed from cross-reactivity, and affinity purified. All antibodies were validated by Western blot analysis, competitive ELISA, and preabsorption experiments. The expression of THIK channels in specific nephron segments was confirmed by double staining with marker proteins. Results indicate that in rat and mouse THIK-1 and THIK-2 were expressed in the proximal tubule (PT), thick ascending limb (TAL), connecting tubule (CNT), and cortical collecting duct (CCD). In human kidney THIK-1 and THIK-2 were localized in PT, TAL and CCD. Immunostaining of rat tissue revealed an intracellular expression of THIK-1 and THIK-2 throughout the identified nephron ...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K TsuchiyaP A Welling
Jan 1, 1990·Pflügers Archiv : European journal of physiology·M BleichR Greger
Oct 1, 1988·Pflügers Archiv : European journal of physiology·J UblH A Kolb
Sep 1, 1993·Pflügers Archiv : European journal of physiology·J Hirsch, E Schlatter
May 1, 1995·The American Journal of Physiology·S BachmannP Mundel
Jan 1, 1995·Pflügers Archiv : European journal of physiology·J Hirsch, E Schlatter
Jun 1, 1994·The Journal of General Physiology·A M HurstP D Bell
Feb 1, 1993·Pflügers Archiv : European journal of physiology·J HirschE Schlatter
Oct 1, 1995·Kidney International·J Hirsch, E Schlatter
Jan 23, 1997·Biochemical and Biophysical Research Communications·C DerstH W Seyberth
Jan 1, 1997·Annual Review of Physiology·C G Nichols, A N Lopatin
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·S Le MaoutP A Welling
Apr 18, 1998·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·A Di StefanoM Wittner
Nov 13, 1998·The Journal of Biological Chemistry·R ReyesM Lazdunski
Dec 9, 1998·The American Journal of Physiology·F CluzeaudN Farman
Aug 24, 1999·The Journal of Physiology·J R HirschE Schlatter
Aug 1, 2000·Reviews of Physiology, Biochemistry and Pharmacology·E KlussmannW Rosenthal
Sep 6, 2000·American Journal of Respiratory Cell and Molecular Biology·C U Cotton
Oct 29, 2000·American Journal of Physiology. Renal Physiology·F Lesage, M Lazdunski
Nov 4, 2000·The Journal of Biological Chemistry·S RajanC Derst
Mar 14, 2001·American Journal of Physiology. Cell Physiology·Y WeiW H Wang
Mar 21, 2001·Nature Reviews. Neuroscience·S A GoldsteinN Zilberberg
Mar 29, 2001·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·J R HirschE Schlatter
Apr 9, 2001·American Journal of Physiology. Renal Physiology·C B WodaL M Satlin
May 18, 2001·Trends in Neurosciences·A J Patel, E Honoré
Jul 28, 2001·Kidney International·G Giebisch
Aug 10, 2001·Current Opinion in Nephrology and Hypertension·C L Huang
Oct 25, 2001·Journal of the American Society of Nephrology : JASN·Franziska TheiligSebastian Bachmann
Dec 6, 2001·Nature·M Hunter
May 1, 2002·The Journal of General Physiology·Diego Alvarez de la RosaCecilia M Canessa
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Rui-Min Gu, Wen-Hui Wang
Jul 24, 2003·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jochen Hirsch, Eberhard Schlatter
Feb 12, 2004·Kidney International·Daniel I LevyDetlef Bockenhauer
Mar 3, 2004·Molecular Interventions·Douglas A BaylissEdmund M Talley

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Citations

Dec 4, 2013·Pflügers Archiv : European journal of physiology·Vijay ReniguntaJürgen Daut
Feb 18, 2012·American Journal of Physiology. Renal Physiology·Kirk L Hamilton, Daniel C Devor
Oct 24, 2014·Pflügers Archiv : European journal of physiology·Delphine BichetFlorian Lesage
Aug 26, 2014·The Journal of Biological Chemistry·Sandy BlinDelphine Bichet
Mar 21, 2015·Pflügers Archiv : European journal of physiology·Vijay ReniguntaJürgen Daut
Apr 16, 2010·Physiological Reviews·Péter Enyedi, Gábor Czirják
Oct 26, 2018·Physiological Reviews·Agustin Gonzalez-VicenteJeffrey L Garvin

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