Cellular localization of TRPC3 channel in rat brain: preferential distribution to oligodendrocytes

Neuroscience Letters
Francesca R FuscoGiorgio Bernardi

Abstract

In the present work we describe the cellular localization of TRPC3 in non-excitable cells as compared to the neurons in normal rat brain. We performed a double labeling study for TRPC3 and one of the following cell-specific markers: mouse anti-glial fibrillary acidic protein (GFAP) for astrocytes; mouse anti-RIP for oligodendrocytes, or mouse anti-OX42 for microglia, or mouse anti-NeUN for neuronal nuclei or mouse anti-tyrosine hydroxylase (TH) for detection of dopaminergic neurons of the substantia nigra. Our double label immunofluorescence study showed that that TRPC3 is mainly localized in oligodendrocytes. These result were confirmed by the electron microscopy study, which showed TRPC3 immunoreactivity in oligodendrocytes. Consistent with the evidence that calcium homeostasis is important to oligodendrocytes for development, myelination, and demyelination [Microsc. Res. Tech. 52 (2001) 672], we can speculate that the distribution of TRPC3 in oligodendrocytes plays a role in myelination and or demyelination processes.

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Citations

Nov 8, 2008·Histochemistry and Cell Biology·Sylvie BoisseauAlexandre Bouron
Mar 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pablo M PaezAnthony T Campagnoni
Mar 9, 2007·Cell and Tissue Research·Sabrina ZechelOliver von Bohlen Und Halbach
Aug 29, 2007·Neuroscience Letters·Carmela GiampàFrancesca R Fusco
Aug 26, 2006·The European Journal of Neuroscience·Alessandro MartoranaFrancesca R Fusco
Feb 19, 2010·Journal of Neurochemistry·Anja HoffmannChristian Harteneck
Jan 27, 2009·Brain Research·Angela S RoeddingJerry J Warsh
Apr 26, 2006·Neuroscience Letters·Zena De MarchFrancesca R Fusco
Dec 17, 2014·Journal of Neuroscience Research·Eric Zhang, Ping Liao
May 1, 2007·Biochimica Et Biophysica Acta·Daniel N CortrightDaniel C Broom
Mar 4, 2020·International Journal of Molecular Sciences·Michael EndersStefanie Kuerten
Mar 2, 2017·Brain Structure & Function·M PapanikolaouA M Butt
Oct 28, 2019·Current Molecular Pharmacology·Melvin N RosalezMarvin A Soriano-Ursúa
Apr 4, 2021·Journal of Clinical Medicine·Alexandru CojocaruEugen Osiac
Jun 19, 2008·Journal of Cell Science·Yilin TaiYizheng Wang

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