Cellular mechanism for potentiation of Ca2+-mediated Cl- secretion by the flavonoid baicalein in intestinal epithelia

The Journal of Biological Chemistry
Grace Gar-Lee YueWing-hung Ko

Abstract

Flavonoids belong to a large group of plant polyphenols that are consumed daily in large amounts. Our previous findings have shown that baicalein, a major flavonoid derived from the medicinal herb Scutellariae radix, induces Cl(-) secretion across rat colonic mucosa. The current study examines the effect of baicalein on Cl(-) secretion in human colonic epithelial (T84) cells and its interaction with Ca(2+)- and cAMP-dependent secretagogues. We have employed a technique that allows concurrent monitoring of short-circuit current (I(SC)) and [Ca(2+)](i) in polarized epithelium. Basolateral application of baicalein induced a concentration-dependent increase in I(SC). The increase in I(SC) was because of Cl(-) secretion and was not accompanied by any discernible increase in [Ca(2+)](i). Baicalein acted synergistically with Ca(2+)- but not cAMP-dependent secretagogues. In the presence of baicalein, the carbachol and histamine induced increases in I(SC) that were markedly potentiated while increases in [Ca(2+)](i) were not significantly enhanced. Baicalein treatment uncoupled Cl(-) secretion from inhibitory effects normally generated by muscarinic activation. Baicalein treatment also resulted in increased cAMP content and activated PK...Continue Reading

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Citations

Nov 18, 2008·American Journal of Respiratory Cell and Molecular Biology·Alison M WongWing-hung Ko
Nov 19, 2013·Pflügers Archiv : European journal of physiology·Jeong Hee HongShmuel Muallem
Oct 4, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Gary LavertySighvatur S Arnason
Jan 22, 2011·Journal of Cellular and Molecular Medicine·Hongguang LiuPradeep K Dudeja
Mar 13, 2014·Cell Calcium·Malini AhujaShmuel Muallem
Aug 26, 2014·International Journal of Endocrinology·Yu FuDongmin Liu
Jun 9, 2006·Biology of Reproduction·Jian-Yang DuWen-Liang Zhou
May 5, 2005·The Journal of Pharmacology and Experimental Therapeutics·Chatsri DeachapunyaNateetip Krishnamra
Jan 12, 2008·The Journal of Pharmacology and Experimental Therapeutics·DaZheng Wu, Zhibi Hu
Apr 3, 2020·Journal of Cellular Physiology·Rui-Gang ZhangWing-Hung Ko
Sep 24, 2005·The Journal of Nutrition·Maximilian SchuierHorst Fischer
Apr 15, 2016·American Journal of Physiology. Cell Physiology·Mei AoMrinalini C Rao
Feb 5, 2021·Biochemistry and Biophysics Reports·Tultul SahaKazi Mirajul Hoque
Sep 18, 2020·Current Pharmaceutical Design·Yong CuiYan Ding

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