Cellular mechanism of melatonin action in neonatal rat pituitary

Neuroendocrinology
J Vanecek

Abstract

Melatonin inhibits gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone (LH) release from neonatal rat anterior pituitary. Melatonin has been shown to decrease the concentration of several second messengers in neonatal pituitary, but it is not known which of them transduces the melatonin effect on LH release. In order to determine the mechanism of melatonin action, we tested the effect of melatonin on GnRH-induced LH release in the presence of specific drugs affecting second messengers. The calcium channel antagonist nifedipine inhibited LH release from cultured pituitary to a similar degree as melatonin and prevented the inhibitory effect of melatonin on LH release. The calcium channel agonist Bay K potentiated the GnRH-stimulated LH release and reduced the inhibitory effect of melatonin on LH release. These data strongly suggest that melatonin inhibits LH release via inhibition of calcium influx through voltage-sensitive channels. The cyclic AMP (cAMP) derivative 8-bromo-cAMP potentiated GnRH stimulation of LH release but did not prevent the melatonin-induced inhibition of LH release. However, when used in combination with Bay K, which reduced only partially the melatonin effect by itself, 8-bromo-cAMP completely...Continue Reading

Citations

Mar 24, 2011·Reproduction, Fertility, and Development·Marcos PedrerosMontserrat Guerra
Apr 29, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chunjin Li, Xu Zhou
May 30, 2014·Zygote : the Biology of Gametes and Early Embryos·Xue-Ming ZhaoHua-Bin Zhu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.