Cellular metabolic activity marker via selective turn-ON detection of transporter protein using nitrobenzoxadiazole-based fluorescent reporter.

Scientific Reports
Tanoy DuttaApurba Koner

Abstract

A nitrobenzoxadiazole-based fluoroprobe (NBD-Bu) is designed to probe cellular metabolic activity in cancer and normal cells. NBD-Bu shows a significant fluorescence enhancement upon selective binding to the transport protein serum albumin in PBS buffer at ambient conditions. Encouraged by this finding, the site- specificity of NBD-Bu has been explored through a competitive displacement assay in the presence of site-specific markers such as warfarin and ibuprofen. Notably, even at micromolar concentrations, the probe possesses the ability to displace the site marker drug ibuprofen, efficiently. Subsequently, high-resolution fluorescence imaging results consolidated the potential of NBD-Bu for detection of abnormal cellular metabolic activity.

References

Jul 16, 1992·Nature·X M He, D C Carter
Jul 23, 2003·Photochemistry and Photobiology·A BarikHari Mohan
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 20, 2005·Journal of Molecular Biology·Jamie GhumanStephen Curry
Jan 6, 2006·IUBMB Life·Mauro FasanoPaolo Ascenzi
Apr 16, 2008·Journal of Combinatorial Chemistry·Young-Hoon AhnYoung-Tae Chang
Aug 22, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yongnian NiSerge Kokot
Nov 26, 2008·The Journal of Biological Chemistry·Ronghua LiuFrances J Sharom
Jun 1, 2011·Nucleic Acids Research·Aurélien GrosdidierOlivier Michielin
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Dec 18, 2013·Carcinogenesis·Thomas N SeyfriedDominic P D'Agostino
Jan 8, 2014·The Journal of Physical Chemistry. B·K BalerI Szleifer
May 14, 2016·Physical Chemistry Chemical Physics : PCCP·Laramie P JamesonSergei V Dzyuba
Dec 19, 2016·Analytical Chemistry·Alana F OgataReginald M Penner
Jun 8, 2017·Bioorganic & Medicinal Chemistry Letters·Shingo SakamotoYasuteru Urano
Sep 1, 2018·Chemical Communications : Chem Comm·Kaushik PalApurba Lal Koner

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Methods Mentioned

BETA
circular
fluorescence
NMR
isothermal titration calorimetry
fluorescence microscopy
confocal microscopy
isothermal titration calorimetric

Software Mentioned

IBH
DAS
ImageJ ( Fiji )
cellSens
PyMOL
Olympus
NanoAnalyze
UCSF Chimera
EADock DSS
SwissDock

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