PMID: 20650149Oct 1, 1995Paper

Cellular neurotoxicity of trivalent manganese bound to transferrin or pyrophosphate studied in human neuroblastoma (SH-SY5Y) cell cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
N SuarezH Eriksson

Abstract

Previous studies have shown that cellular uptake of manganese is related to its binding to transferrin. However, it is not known how transferrin binding influences manganese toxicity. Therefore, the cytotoxic activity of manganese bound as manganic ion to either transferrin or pyrophosphate was investigated in the cloned human neuroblastoma cell line SH-SY5Y. The toxicity of the two compounds was studied as changes in cell growth and survival by lipid and protein determinations. There was a significant difference in the toxicity between the two complexes after 72 hr of exposure. The toxicity of the manganic-pyrophosphate (MnPPi) complex differed from that of the manganic-transferrin (MnTf) complex by a factor of 2 (IC(50): 26 +/- 2.6 and 65 +/- 2.4 mum, respectively). After 3 days of exposure to MnPPi and MnTf, the mitochondrial integrity was monitored by the mitochondrial dehydrogenase activity. The two manganese complexes reduced the enzyme activity to the same extent. Measurements of membrane integrity, using (3)[H]-2-deoxy-d-glucose as a probe, showed an increase in the membrane permeability of cells exposed to MnPPi for 60 min. Exposure to MnTf did not result in any significant change in membrane permeability. These findin...Continue Reading

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Citations

Feb 12, 2013·Free Radical Biology & Medicine·Ebany J Martinez-FinleyThomas E Gunter
Jul 26, 2011·Biochimica Et Biophysica Acta·John B Vincent, Sharifa Love
Mar 1, 2013·Toxicology Research·April P Neal, Tomas R Guilarte
May 1, 2001·Current Protocols in Toxicology·M Ehrich, L Sharova

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