Cellular organization and substructure measured using angle-resolved low-coherence interferometry

Biophysical Journal
Adam WaxM S Feld

Abstract

We measure the organization and substructure of HT29 epithelial cells in a monolayer using angle-resolved low-coherence interferometry. This new technique probes cellular structure by measuring scattered light, as in flow cytometry, but offers an advantage in that the structure can be examined in situ, avoiding the need to disrupt the cell monolayer. We determine the size distribution of the cell nuclei by fitting measured light-scattering spectra to the predictions of Mie theory. In addition, we obtain information about the cellular organization and substructure by examining the spatial correlations within the monolayer. A remarkable finding is that the spatial correlations over small length scales take the form of an inverse power law, indicating the fractal nature of the packing of the subcellular structures. We also identify spatial correlations on a scale large compared with the size of a cell, indicating an overlying order within the monolayer.

References

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Citations

Jan 29, 2003·Journal of Cellular Biochemistry. Supplement·Karen E Shafer-PeltierMichael S Feld
Jul 26, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Michael P SchwartzMichael J Sailor
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hariharan SubramanianVadim Backman
Sep 8, 2011·Journal of Biomedical Optics·David D NolteKwan Jeong
Oct 28, 2011·Journal of Biomedical Optics·Neil TerryAdam Wax
May 7, 2009·Theoretical Biology & Medical Modelling·Alexei Kurakin
Jun 8, 2013·The Review of Scientific Instruments·Oleksandra V LyulkoDavid J Brenner
May 2, 2009·Journal of Biomedical Optics·Condon LauMichael Feld
Apr 5, 2011·Journal of Biomedical Optics·Nienke BosschaartMaurice C G Aalders
Dec 5, 2015·Biophysical Journal·Ji YiVadim Backman
Jul 12, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Cherry GreinerIrene Georgakoudi
Jun 4, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Cherry GreinerIrene Georgakoudi
Aug 5, 2010·Biophysical Journal·Dhwanil DamaniaVadim Backman
Jan 18, 2005·Biophysical Journal·Jeremy D WilsonThomas H Foster
Apr 8, 2015·PLoS Computational Biology·Nancy K DrewAnna Grosberg
Oct 4, 2005·Optics Letters·Jeremy D Wilson, Thomas H Foster
Sep 27, 2018·Journal of Biomedical Optics·Mohammad NaserNada N Boustany
Oct 28, 2016·Journal of Biomechanical Engineering·Nancy K DrewAnna Grosberg
Jun 23, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Kevin J ChalutKam W Leong
Mar 9, 2007·Journal of Biomedical Optics·Jeremy D WilsonThomas H Foster
Mar 31, 2005·Physics in Medicine and Biology·Dzena Hidović-Rowe, Ela Claridge
Nov 13, 2007·Journal of Biomedical Optics·Wendy A WellsJames M Crawford
Jan 11, 2019·Physics in Medicine and Biology·Dennis MüllerAlwin Kienle
Dec 10, 2009·Optics Express·Maxim KalashnikovMichael S Feld
Feb 8, 2011·Soft Matter·Kevin J ChalutKam W Leong
Dec 31, 2005·Physical Review Letters·Erwan Baleine, Aristide Dogariu
Jun 29, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·P ChelminiakR E Marsh

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