Cellular origin of microRNA-371a-3p in healthy males based on systematic urogenital tract tissue evaluation

Andrology
W P A BoellaardL H J Looijenga

Abstract

The microRNA-371a-3p (miR-371a-3p) has been reported to be an informative liquid biopsy (serum and plasma) molecular biomarker for both diagnosis and follow-up of patients with a malignant (testicular) germ cell tumor ((T)GCT). It is expressed in all histological cancer elements, with the exception of mature teratoma. However, normal testis, semen, and serum of males with a disrupted testicular integrity without a TGCT may contain miR-371a-3p levels above threshold, of which the cellular origin is unknown. Therefore, a series of relevant tissues (frozen and formalin-fixed paraffin-embedded (FFPE), when available) from the complete male urogenital tract (i.e., kidney to urethra and testis to urethra) and semen was investigated for miR-371a-3p levels using targeted quantitative RT-PCR (qRT-PCR). In total, semen of males with normospermia (n = 11) and oligospermia (n = 3) was investigated, as well as 88 samples derived from 32 different patients. The samples represented one set of tissues related to the entire male urogenital tract (11 anatomical locations), three sets for 10 locations, and four sets for six locations. All testis parenchyma (n = 17) cases showed low miR-371a-3p levels. Eight out of 14 (57%) semen samples showed de...Continue Reading

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Citations

Mar 12, 2020·Nature Reviews. Urology·Kristian AlmstrupKlaus-Peter Dieckmann
Oct 30, 2019·International Journal of Molecular Sciences·Leendert H J LooijengaMuhammad T Idrees
Mar 20, 2020·Journal of Assisted Reproduction and Genetics·Yousef DaneshmandpourEbrahim Sakhinia
Apr 4, 2021·International Journal of Molecular Sciences·João LoboRui Henrique

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