Cellular pathways controlling integron cassette site folding.

The EMBO Journal
Céline LootD Mazel

Abstract

By mobilizing small DNA units, integrons have a major function in the dissemination of antibiotic resistance among bacteria. The acquisition of gene cassettes occurs by recombination between the attI and attC sites catalysed by the IntI1 integron integrase. These recombination reactions use an unconventional mechanism involving a folded single-stranded attC site. We show that cellular bacterial processes delivering ssDNA, such as conjugation and replication, favour proper folding of the attC site. By developing a very sensitive in vivo assay, we also provide evidence that attC sites can recombine as cruciform structures by extrusion from double-stranded DNA. Moreover, we show an influence of DNA superhelicity on attC site extrusion in vitro and in vivo. We show that the proper folding of the attC site depends on both the propensity to form non-recombinogenic structures and the length of their variable terminal structures. These results draw the network of cell processes that regulate integron recombination.

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Citations

Jun 29, 2012·Nucleic Acids Research·Céline LootDidier Mazel
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·David BikardDidier Mazel
Aug 17, 2010·Annual Review of Genetics·Guillaume CambrayDidier Mazel
Apr 22, 2015·Journal of Bacteriology·Olivier Barraud, Marie-Cécile Ploy
May 23, 2014·Microbiology and Molecular Biology Reviews : MMBR·Michael R Gillings
Jul 4, 2019·MBio·Veronica NegroZeynep Baharoglu
Dec 20, 2018·Nucleic Acids Research·Ann MukhortavaMichael Schlierf
May 29, 2021·Nucleic Acids Research·Claire VitCéline Loot
Jun 25, 2015·Microbiology Spectrum·José Antonio EscuderoDidier Mazel

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