Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2 γ in Phospholipid Remodeling

Cells
Patricia LebreroJesús Balsinde

Abstract

Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis. AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens. In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108. All three strains remodeled AA between phospholipids with similar magnitude and kinetics, thus demonstrating that cellular plasmalogen content does not influence the process. Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process. Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect. These results uncove...Continue Reading

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Citations

Oct 23, 2020·Biomolecules·Makoto MurakamiYoshitaka Taketomi
Dec 6, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin SchwarzCatharine M Bosio
Aug 31, 2020·Neurobiology of Disease·Fabian DorningerJohannes Berger
May 7, 2021·Medicinal Research Reviews·Qing-Zhang TuoPeng Lei
Oct 12, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Daiki HayashiEdward A Dennis

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Methods Mentioned

BETA
transacylation
lavage
Protein Assay
PCR
transfection

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