Cellular prion protein is expressed in a subset of neuroendocrine cells of the rat gastrointestinal tract

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Z MarcosL Guembe

Abstract

Prion diseases are believed to develop from the conformational change of normal cellular prion protein (PrPc) to a pathogenic isoform (PrPsc). PrPc is present in both the central nervous system and many peripheral tissues, although protein concentration is significantly lower in non-neuronal tissues. PrPc expression is essential for internalization and replication of the infectious agent. Several works have pointed to the gastrointestinal (GI) tract as the principal site of entry of PrPsc, but how passage through the GI mucosa occurs is not yet known. Here we studied PrPc expression using Western blot, RT-PCR, and immunohistochemistry in rat GI tract. PrPc mRNA and protein were detected in corpus, antrum, duodenum, and colon. Immunoreactivity was found in scattered cells of the GI epithelium. With double immunofluorescence, these cells have been identified as neuroendocrine cells. PrPc immunostaining was found in subsets of histamine, somatostatin (Som), ghrelin, gastrin (G), and serotonin (5HT) cells in stomach. In small and large bowel, PrPc cells co-localized with subpopulations of 5HT-, Som-, G-, and peptide YY-immunolabeled cells. Our results provide evidence for a possible and important role of endocrine cells in the inte...Continue Reading

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Citations

Oct 30, 2007·Biochemical and Biophysical Research Communications·Li LiNeil R Cashman
May 14, 2005·Annals of the New York Academy of Sciences·Z MarcosL Guembe
May 14, 2005·Annals of the New York Academy of Sciences·Z MarcosL Guembe
Sep 15, 2007·Brain Research·Francisco J Moleres, José L Velayos
Aug 20, 2005·Brain Research·Francisco J Moleres, José L Velayos
Sep 20, 2011·The American Journal of Pathology·Gary R MartinFrank R Jirik
Aug 3, 2018·Nature Reviews. Gastroenterology & Hepatology·Keith A SharkeyDerek M McKay

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