Cellular regulation of ADP-ribosylation of proteins. III. Selective augmentation of in vitro ADP-ribosylation of histone H3 in murine thymic cells after in vivo emetine treatment

Experimental Cell Research
A Soóki-TóthE Kun

Abstract

Thymic cells were isolated at intervals of between 0 and 144 h from mice that received one intraperitoneal injection of emetine (33 mg/kg), and thymus weight, incorporation of [14C]leucine into proteins and [3H]thymidine into DNA in intact thymic cells, as well as initial rates of protein ADP-ribosylation in permeabilized cells [A. Sóoki-Tóth, F. Asghari, E. Kirsten, and E. Kun (1987) Exp. Cell Res. 170, 93] were simultaneously monitored. The effect of emetine as an inhibitor of protein synthesis [F. Antoni, N. G. Luat, I. Csuka, I. Oláh, A. Sóoki-Tóth, and G. Bánfalvi (1987) Int. J. Immunopharmacol. 9, 333] corresponds to the induction of sequential cellular events, such as cell exit and remigration, by other antimitotic agents [C. Penit and F. Vasseur (1988) J. Immunol. 140, 3315] and produces an activation of proliferation of cells reentering into this organ. Proliferation, as demonstrated by a large increase in DNA synthesis and entrance into S phase, was kinetically related to an apparent increase in poly(ADP-ribose) polymerase activity in thymic cells and a highly significant in vitro ADP-ribosylation of histone H3. Since no DNA fragmentation occurred in thymic cells, as tested by a fluorometric technique [C. Birnboim and...Continue Reading

References

Sep 2, 1975·Biochemical and Biophysical Research Communications·E G Miller
Dec 13, 1979·Nature·H Juarez-SalinasM K Jacobson
Jan 1, 1979·Annual Review of Biochemistry·I Isenberg
Aug 15, 1985·The Biochemical Journal·J C Gaal, C K Pearson
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A TsengE Kun
Jul 1, 1988·Immunology Today·M A RitterH J Schuurman
May 2, 1988·European Journal of Biochemistry·J Wesierska-Gadek, G Sauermann
Jan 1, 1988·Developmental and Comparative Immunology·I OláhI Töró
Jan 1, 1987·International Journal of Immunopharmacology·F AntoniG Bánflavi
Jan 1, 1985·Annual Review of Biochemistry·K Ueda, O Hayaishi
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley
Mar 15, 1984·European Journal of Biochemistry·G BanfalviF Antoni
Jan 1, 1982·Progress in Nucleic Acid Research and Molecular Biology·P MandelC Niedergang
Jan 1, 1983·Advances in Enzyme Regulation·E KunG Milo
Dec 7, 1983·Journal of Theoretical Biology·J C Stockert
Jul 1, 1984·Biochemical Pharmacology·I Csuka, F Antoni
Apr 1, 1980·Biochemical Society Transactions·M R PurnellW J Whish
May 20, 1983·Biochimica Et Biophysica Acta·C S Surowy, N A Berger
Dec 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A P Grollman

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