Cellular-resolution 3D virtual histology of human coronary arteries using x-ray phase tomography

Scientific Reports
William VågbergH M Hertz

Abstract

High-spatial-resolution histology of coronary artery autopsy samples play an important role for understanding heart disease such as myocardial infarction. Unfortunately, classical histology is often destructive, has thick slicing, requires extensive sample preparation, and is time-consuming. X-ray micro-CT provides fast nondestructive 3D imaging but absorption contrast is often insufficient, especially for observing soft-tissue features with high resolution. Here we show that propagation-based x-ray phase-contrast tomography has the resolution and contrast to image clinically relevant soft-tissue features in intact coronary artery autopsy samples with cellular resolution. We observe microscopic lipid-rich plaques, individual adipose cells, ensembles of few foam cells, and the thin fibrous cap. The method relies on a small-spot laboratory x-ray microfocus source, and provides high-spatial resolution in all three dimensions, fast data acquisition, minimum sample distortion and requires no sample preparation.

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Citations

Sep 27, 2018·Radiology·Jenny RomellHans M Hertz
Mar 3, 2020·Journal of Medical Imaging·Marina EckermannTim Salditt
Jul 8, 2020·International Journal of Molecular Sciences·Samantha J BorlandAnn E Canfield
Jul 25, 2019·Scientific Reports·Alessandra GiulianiPasquapina Ciarmela
Nov 5, 2020·Journal of Synchrotron Radiation·Jasper FrohnTim Salditt
Oct 30, 2020·Optics Express·Hanna Dierks, Jesper Wallentin
Nov 18, 2020·Radiology·Alan C KwanJoão A C Lima
Dec 11, 2020·Scientific Reports·Vanessa Isabell JurtzCamilla Ingvorsen
Apr 28, 2021·Proceedings of the National Academy of Sciences of the United States of America·Daniel KeppelerTobias Moser
Jun 25, 2021·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Alessandro Olivo

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Methods Mentioned

BETA
x-ray tomography
x-ray
x-ray phase
x-ray imaging

Software Mentioned

Octopus Reconstruction

Related Concepts

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