PMID: 16502650Mar 1, 2006Paper

Cellular response of adipose derived passage-4 adult stem cells to freezing stress

Journal of Biomechanical Engineering
Ram V DevireddyJeffrey M Gimble

Abstract

A differential scanning calorimeter technique was used to generate experimental data for volumetric shrinkage during cooling at 20 degrees C/min in adipose derived adult stem cells (ASCs) in the presence and absence of cryoprotective agents (CPAs). By fitting a model of water transport to the experimentally determined volumetric shrinkage data, the membrane permeability parameters of ASCs were obtained. For passage-4 (P4) ASCs, the reference hydraulic conductivity Lpg and the value of the apparent activation energy ELP were determined to be 1.2 X 10(-13) m3/Ns and 177.8 kJ/mole, respectively. We found that the addition of either glycerol or dimethylsulfoxide (DMSO) significantly decreased the value of the reference hydraulic conductivity Lpg(cpa) and the value of the apparent activation energy ELp(cpa) in P4 ASCs. The values of Lpg(cpa) in the presence of glycerol and DMSO were determined as 0.39 x 10(-13) and 0.50 X 109-13) m3/Ns, respectively, while the corresponding values of ELp(cpa) were 51.0 and 61.5 kJ/mole. Numerical simulations of water transport were then performed under a variety of cooling rates (5-100 degreesC/min) using the experimentally determined membrane permeability parameters. And finally, the simulation res...Continue Reading

References

Aug 1, 1997·Journal of Biomechanical Engineering·P V Pazhayannur, J C Bischof
Jul 21, 1999·Journal of Biomechanical Engineering·D J SmithJ C Bischof
Jun 2, 2000·Science·D L ClarkeJ Frisén
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lijun YangAmmon B Peck
Mar 3, 2004·Molecular Reproduction and Development·R V DevireddyS P Leibo
Apr 29, 2005·Journal of Microscopy·S Yuan, K R Diller
Jun 24, 2005·Journal of Biomechanical Engineering·Sreedhar Thirumala, Ram V Devireddy

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Citations

Feb 24, 2010·Annals of Biomedical Engineering·Avishek Guha, Ram Devireddy
Oct 21, 2009·Tissue Engineering. Part C, Methods·Sreedhar ThirumalaRam V Devireddy
Dec 8, 2009·Journal of Tissue Engineering and Regenerative Medicine·Sreedhar ThirumalaRam V Devireddy
Jan 10, 2016·Injury·Pamela KuehlfluckUNKNOWN HTRG-Heidelberg Trauma Research Group
May 31, 2013·Journal of Biomechanical Engineering·Angela SeawrightBumsoo Han
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Brian C GohJeffrey M Gimble
Jan 5, 2010·Organogenesis·Sreedhar ThirumalaErik J Woods
May 29, 2018·Scientific Reports·Shahensha ShaikRam Devireddy

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