Cellular Response of Stem Cells on Nanofibrous Scaffold for Ocular Surface Bioengineering

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Alireza Baradaran-RafiiSaeed Heidari-Keshel

Abstract

In this study, the human limbus stem cell was used to evaluate the phenotypic profile, viability, proliferation, and attachment ability of nanofibrous poly (3-hydroxybutyrate-co-3-hydroxyvalerate) substrates. Results indicated that the all substrates were highly biocompatible, as firmly anchored to the substrates, and were able to retain a normal corneal stem cell phenotype. Immunocytochemistry results revealed no change in the expression profile of cells grown on nanofibrous substrate when compared with those grown on amniotic membrane. In addition, nanofibrous substrate provides not only a milieu supporting cells expansion, but also serve as a useful alternative carrier for ocular surface engineering.

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Nov 1, 2013·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Esmaeil Biazar, Saeed Heidari Keshel

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Citations

May 25, 2016·Preparative Biochemistry & Biotechnology·A P BonartsevG A Bonartseva
Apr 20, 2018·Journal of Medical Engineering & Technology·Esmaeil BiazarDariush Biazar
Feb 2, 2018·Advanced Healthcare Materials·Rachel WilliamsHannah Levis
Jan 18, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Maryam EbrahimiSaeed Heidari-Keshel
Jul 23, 2020·Tissue Engineering and Regenerative Medicine·S Sharareh MahdaviAli R Djalilian

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