PMID: 2484391Apr 1, 1989Paper

Cellular selenoproteins and the effects of selenite on cell proliferation

Biological Trace Element Research
J A Golczewski, G D Frenkel

Abstract

The incorporation of radioactive selenium into cellular proteins and the effect of selenite on proliferation were examined in human (HeLa, HT-29, and IMR-90) and mouse (3T3 and CMT-93) cell lines. Proteins incorporating selenium were detected by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Major polypeptide subunits at 60, 23, 21, 19, and 16 kD were detected in the two tumorigenic and one normal human cell lines. The 23 kD polypeptide migrated to the same position on the gel as the major subunit of human erythrocyte glutathione peroxidase. In the mouse cells, the 60 kD polypeptide was almost entirely absent; four other major selenoproteins were detected, with molecular weights similar to those in the human cells. In both mouse and human cells, the same pattern of selenoproteins was observed irrespective of whether the cells were grown in medium containing 10% fetal bovine serum or in defined medium supplemented with 0.1 or 1 microM selenite, or with 1% serum. The effect of selenite on proliferation of HeLa, HT-29, and CMT-93 cells in medium supplemented with 10% fetal bovine serum and in serum-free medium was examined. At concentrations up to about 1 microM, selenite stimulated proliferation of the...Continue Reading

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Citations

Nov 1, 1993·Biological Trace Element Research·C MacVicar, G D Frenkel
Jan 19, 1999·Biological Trace Element Research·P B CaffreyG D Frenkel
Jul 18, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fusako MaehiraYukinori Eguchi
Feb 27, 2007·The Journal of Nutritional Biochemistry·Naveen Kaushal, Mohinder P Bansal
Sep 22, 2005·Endocrine Reviews·J KöhrleJ E Dumont
Jun 11, 2004·Medical and Veterinary Entomology·H Al-TalafhaA Katbeh Bader
Apr 1, 1992·Biological Trace Element Research·G N Schrauzer
Apr 2, 2002·The Journal of Nutrition·Huawei Zeng

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