Cellular self-assembly into 3D microtissues enhances the angiogenic activity and functional neovascularization capacity of human cardiopoietic stem cells

Angiogenesis
Petra WolintMaximilian Y Emmert

Abstract

While cell therapy has been proposed as next-generation therapy to treat the diseased heart, current strategies display only limited clinical efficacy. Besides the ongoing quest for the ideal cell type, in particular the very low retention rate of single-cell (SC) suspensions after delivery remains a major problem. To improve cellular retention, cellular self-assembly into 3D microtissues (MTs) prior to transplantation has emerged as an encouraging alternative. Importantly, 3D-MTs have also been reported to enhance the angiogenic activity and neovascularization potential of stem cells. Therefore, here using the chorioallantoic membrane (CAM) assay we comprehensively evaluate the impact of cell format (SCs versus 3D-MTs) on the angiogenic potential of human cardiopoietic stem cells, a promising second-generation cell type for cardiac repair. Biodegradable collagen scaffolds were seeded with human cardiopoietic stem cells, either as SCs or as 3D-MTs generated by using a modified hanging drop method. Thereafter, seeded scaffolds were placed on the CAM of living chicken embryos and analyzed for their perfusion capacity in vivo using magnetic resonance imaging assessment which was then linked to a longitudinal histomorphometric ex v...Continue Reading

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Citations

Jun 20, 2019·Journal of Cellular Physiology·Xiuling ShangRongguo Yu
Mar 30, 2020·Tissue Engineering. Part B, Reviews·Greet MerckxAnnelies Bronckaers
Jul 24, 2020·Cells·Michele Filippo BuonoBramasta Nugraha
Mar 21, 2020·Npj Regenerative Medicine·D Kent ArrellAndre Terzic
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Ting XinFang Hu
Oct 7, 2020·Journal of Receptor and Signal Transduction Research·Qiubei LuBinhao Wang
Oct 10, 2020·Frontiers in Cardiovascular Medicine·Peter ZillaTim Pennel
Oct 25, 2020·Stem Cells Translational Medicine·Mark LiAtta Behfar
Feb 14, 2021·Npj Regenerative Medicine·D Kent ArrellAndre Terzic

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Clinical Trials Mentioned

NCT00810238
NCT01768702
NCT02317458

Software Mentioned

view2
StatView
excel
ImageJ
NDP

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