Cellular Shuttles: Monocytes/Macrophages Exhibit Transendothelial Transport of Nanoparticles under Physiological Flow

ACS Applied Materials & Interfaces
Thomas L MooreRuth Lyck

Abstract

A major hurdle in the development of biomedical nanoparticles (NP) is understanding how they interact with complex biological systems and navigate biological barriers to arrive at pathological targets. It is becoming increasingly evident that merely controlling particle physicochemical properties may not be sufficient to mediate particle biodistribution in dynamic environments. Thus, researchers are increasingly turning toward more complex but likewise more physiological in vitro systems to study particle--cell/particle-system interactions. An emerging paradigm is to utilize naturally migratory cells to act as so-called "Trojan horses" or cellular shuttles. We report here the use of monocytes/macrophages to transport NP across a confluent endothelial cell layer using a microfluidic in vitro model. With a custom-built flow chamber, we showed that physiological shear stress, when compared to low flow or static conditions, increased NP uptake by macrophages. We further provided a mathematical explanation for the effect of flow on NP uptake, namely that the physical exposure times of NP to cells is dictated by shear stress (i.e., flow rate) and results in increased particle uptake under flow. This study was extended to a multicellu...Continue Reading

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Citations

May 16, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ana B García-MartínRuth Lyck
Mar 21, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Nahal HabibiJoerg Lahann
Apr 8, 2019·Biomedical Microdevices·Valeria LusiPaolo Decuzzi
Dec 14, 2018·Frontiers in Bioengineering and Biotechnology·Antonio CervadoroStefano Luin
Aug 29, 2019·Biomolecules·Armita Mahdavi GorabiAmirhossein Sahebkar
Apr 3, 2021·Drug Delivery and Translational Research·Justin SilbermanErika Moore
Aug 16, 2018·ACS Applied Materials & Interfaces·Molly K ShaveMaria M Santore

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