Cellular Targeting of Bispecific Antibody-Functionalized Poly(ethylene glycol) Capsules: Do Shape and Size Matter?

ACS Applied Materials & Interfaces
Danzi SongFrank Caruso

Abstract

In the present study, a capsule system that consists of a stealth carrier based on poly(ethylene glycol) (PEG) and functionalized with bispecific antibodies (BsAbs) is introduced to examine the influence of the capsule shape and size on cellular targeting. Hollow spherical and rod-shaped PEG capsules with tunable aspect ratios (ARs) of 1, 7, and 18 were synthesized and subsequently functionalized with BsAbs that exhibit dual specificities to PEG and epidermal growth factor receptor (EGFR). Dosimetry (variation between the concentrations of capsules present and capsules that reach the cell surface) was controlled through "dynamic" incubation (i.e., continuously mixing the incubation medium). The results obtained were compared with those obtained from the "static" incubation experiments. Regardless of the incubation method and the capsule shape and size studied, BsAb-functionalized PEG capsules showed >90% specific cellular association to EGFR-positive human breast cancer cells MDA-MB-468 and negligible association with both control cell lines (EGFR negative Chinese hamster ovary cells CHO-K1 and murine macrophages RAW 264.7) after incubation for 5 h. When dosimetry was controlled and the dose concentration was normalized to the ...Continue Reading

References

Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L W SeymourR Duncan
Mar 18, 1994·Science·R GrefR Langer
Sep 10, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Chao FangHong-Zhuan Chen
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Julie A Champion, Samir Mitragotri
Apr 13, 2006·Nano Letters·B Devika ChithraniWarren C W Chan
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stephanie E A GrattonJoseph M DeSimone
Jul 17, 2009·Advanced Materials·Sulin ZhangSubra Suresh
Apr 14, 2010·PloS One·Nishit Doshi, Samir Mitragotri
Sep 11, 2010·Nature Biotechnology·Gary Walsh
Dec 2, 2010·Particle and Fibre Toxicology·Paul M HinderliterJustin G Teeguarden
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Timothy J MerkelJoseph M DeSimone
Jan 22, 2011·Journal of the American Chemical Society·Anke KuijkArnout Imhof
Apr 26, 2011·Nature Nanotechnology·Eun Chul ChoYounan Xia
Jun 23, 2011·Small·Jin WangJoseph M DeSimone
Jun 23, 2011·Small·Poornima KolharSamir Mitragotri
Nov 3, 2011·Chemical Communications : Chem Comm·Damien MertzFrank Caruso
Jan 28, 2012·Chemical Society Reviews·Stefaan De KokerBruno G De Geest
Apr 25, 2012·Annual Review of Biomedical Engineering·Alexandre AlbaneseWarren C W Chan
May 23, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Farrell R KerseyJoseph M DeSimone
Jul 28, 2012·Therapeutic Delivery·Yaling LiuVladimir R Muzykantov
Dec 14, 2012·ACS Nano·Olga ShimoniFrank Caruso
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sutapa BaruaSamir Mitragotri
Feb 18, 2014·International Journal of Nanomedicine·Vanna SannaMario Sechi
Feb 3, 2015·Advanced Healthcare Materials·Sourabh ShuklaNicole F Steinmetz
Sep 1, 2015·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Brittany L BanikJustin L Brown
Sep 5, 2015·Journal of Nanobiotechnology·Bogdan ParakhonskiyAndre G Skirtach
May 21, 2016·Accounts of Chemical Research·Jiwei CuiFrank Caruso
May 29, 2016·Cancer Letters·Yong XinGuan Jiang
Jun 11, 2016·Advanced Healthcare Materials·Christopher B HowardKristofer J Thurecht
Jul 8, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Jiwei CuiFrank Caruso
Oct 18, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Mattias BjörnmalmFrank Caruso
Nov 12, 2016·Nature Reviews. Cancer·Jinjun ShiOmid C Farokhzad
May 27, 2017·Bioconjugate Chemistry·Huanli SunFrank Caruso

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Engineering

Antibody engineering technologies are constantly advancing to improve the clinical effectiveness of monoclonal and bispecific antibodies. Discover the latest research on Antibody Engineering here.