Cellular targets and receptors for interleukin-6. I. In vivo and in vitro uptake of IL-6 in liver and hepatocytes

European Journal of Clinical Investigation
O SonneC Munck Petersen

Abstract

Interleukin-6 (IL-6) is a potent stimulator of the hepatic synthesis of acute-phase proteins. 125I-labelled IL-6 disappeared from the blood of rats with an overall half-time of about 1.5 min; 41% of the injected tracer dose was recovered in the liver by 15 min. The clearance was biphasic. The simultaneous injection of tracer and an excess of unlabelled IL-6 eliminated the initial rapid phase, and reduced the hepatic uptake to 14%. Light microscopic autoradiography showed 5% of the grains over non-hepatocytes, and 80% over hepatocytes, accumulating in areas around the bile canaliculi. Thereafter, degradation products accumulated in the bile. At 4 degrees C, isolated rat hepatocytes bound IL-6 with an apparent Kd of 39 pmol l-1 to a uniform class of 4500 receptors per cell with an apparent molar mass of 115-120 kg mol-1. The HepG2 human hepatocellular cell line bound IL-6 with an apparent Kd of 21 pmol l-1 to a uniform class of 1200 receptors per cell with an apparent molar mass of 155-160 kg mol-1. At 37 degrees C, both cell types endocytosed the bound ligand slowly, and degradation products appeared in the medium after a relatively long lag period (40 min in hepatocytes and 1 h in HepG2 cells).

References

Dec 1, 1977·Experimental Cell Research·H PertoftB Klingeborn
Jan 23, 1986·Biochimica Et Biophysica Acta·J Gliemann, O Davidsen
Nov 1, 1986·The Journal of Clinical Investigation·D H PerlmutterH R Colten
May 1, 1988·European Journal of Immunology·T GeigerP C Heinrich
Oct 1, 1989·European Journal of Immunology·C M PetersenB Møller
Oct 1, 1988·Physiological Reviews·O Sonne
Jul 1, 1988·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T Kishimoto, T Hirano
Aug 1, 1988·Human Genetics·G R SutherlandA F Lopez
Jan 1, 1983·Annals of the New York Academy of Sciences·L Sottrup-JensenS Magnusson
Jan 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·O SonneJ Markussen

❮ Previous
Next ❯

Citations

Dec 1, 1995·British Journal of Haematology·T Lavabre-BertrandJ Brochier
Jul 15, 2003·American Journal of Physiology. Endocrinology and Metabolism·Mark A FebbraioBente Klarlund Pedersen
Jul 18, 2018·Journal of Molecular Cell Biology·Daniëlle R J VerboogenGeert van den Bogaart
Oct 1, 1992·Leukemia & Lymphoma·C W HollenS A Burstein
Jun 3, 2005·The American Journal of Gastroenterology·Jaana LatvalaOnni Niemelä
May 16, 2000·Critical Reviews in Clinical Laboratory Sciences·R E Banks
Oct 1, 1991·British Journal of Haematology·C W HollenS A Burstein
Dec 1, 1995·Journal of Critical Care·C M PastorD M Payen
Sep 1, 1992·Research in Immunology·J GauldieH Baumann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.