Cellular targets and receptors for interleukin-6. II. Characterization of IL-6 binding and receptors in peripheral blood cells and macrophages

European Journal of Clinical Investigation
C Munck PetersenB K Møller

Abstract

Within 15 min, approximately 2.5% of 125I-labelled interleukin-6 (IL-6) injected intravenously into rats was taken up by the spleen. As determined by light microscopic autoradiography, uptake was mainly (60%) accounted for by macrophages in the red pulp. 125I-IL-6 binding in rat peritoneal macrophages was quantitatively similar to that in cultured human monocytes and T-cells. By comparison, IL-6 binding to polymorphonuclear granulocytes and freshly isolated monocytes was low. Stimulation with antigen, but not with mitogen (PWM), induced receptor presentation in B-cells, whereas antigen and mitogen downregulated the binding in T-cells. At 4 degrees C, labelled IL-6 bound to cells with a half-time of about 1.5 h. Binding appeared reversible, but dissociation was slow and incomplete. The apparent Kd for IL-6 binding was about 30 pmol l-1 in most cell types, however, values of approximately 120 pmol l-1 were obtained in polymorphonuclear granulocytes. At 37 degrees C, 125I-IL-6 was rapidly internalized by T-cells and monocyte-macrophages, and after a lag time, TCA-soluble radioactivity was released from the cells following a sigmoidal curve. Polyacrylamide gel electrophoresis of radiolabelled IL-6 cross-linked to its binding sites ...Continue Reading

References

Aug 1, 1990·European Journal of Clinical Investigation·O SonneC Munck Petersen
Feb 1, 1988·The Journal of Experimental Medicine·A MuraguchiT Kishimoto
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R D GarmanD H Raulet
Jan 1, 1968·Vox Sanguinis·A Svejgaard, F Kissmeyer-Nielsen

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Citations

Nov 1, 1991·American Journal of Respiratory Cell and Molecular Biology·V L Shepherd
Aug 1, 1990·European Journal of Clinical Investigation·O SonneC Munck Petersen
Jan 3, 2001·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·P BongioanniG Meucci
Jan 6, 2000·Acta Orthopaedica Scandinavica·S D Neale, N A Athanasou
Jul 11, 2000·The European Journal of Neuroscience·P G MurphyP M Richardson
Jun 1, 1992·Antiviral Research·D K MeijerG Molema
Jul 1, 1992·Baillière's Clinical Haematology·O KanA D Whetton

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