Cellular titration of apoptosis with steady state concentrations of H(2)O(2): submicromolar levels of H(2)O(2) induce apoptosis through Fenton chemistry independent of the cellular thiol state

Free Radical Biology & Medicine
F Antunes, E Cadenas

Abstract

Apoptosis was studied under conditions that mimic the steady state of H(2)O(2) in vivo. This is at variance with previous studies involving a bolus addition of H(2)O(2), a procedure that disrupts the cellular homeostasis. The results allowed us to define three phases for H(2)O(2)-induced apoptosis in Jurkat T-cells with reference to cytosolic steady state concentrations of H(2)O(2) [(H(2)O(2))(ss)]: (H(2)O(2))(ss) values below 0.7 microM elicited no effects; (H(2)O(2))(ss) approximately 0.7-3 microM induced apoptosis; and (H(2)O(2))(ss) > 3 microM yielded no additional apoptosis and a gradual shift towards necrosis as the mode of cell death were observed. H(2)O(2)-induced apoptosis was not affected by either BCNU, an inhibitor of glutathione reductase, or diamide, a compound that reacts both with low-molecular weight and protein thiols, or selenols. Glutathione depletion, accomplished by incubating cells either with buthionine sulfoximine or in cystine-free medium, rendered cells more sensitive to H(2)O(2)-induced apoptosis, but did not change the threshold and saturating concentrations of H(2)O(2) that induced apoptosis. Two unrelated metal chelators, desferrioxamine and dipyridyl, strongly protected against H(2)O(2)-induced a...Continue Reading

References

Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J Smith, A Shrift
Sep 15, 1975·European Journal of Biochemistry·H Sies, K H Summer
Apr 1, 1991·Differentiation; Research in Biological Diversity·G B PierceA L Lewellyn
May 1, 1991·Biochemical Pharmacology·J B LloydC Rice-Evans
Feb 1, 1990·The Biochemical Journal·G A MurrellL Bromley
Mar 17, 1988·Biochimica Et Biophysica Acta·K Olafsdottir, D J Reed
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A NovogrodskyK H Stenzel
Apr 1, 1995·Free Radical Biology & Medicine·R H Burdon
Jan 1, 1997·Free Radical Biology & Medicine·Y J SuzukiA Sevanian
Jan 1, 1997·European Journal of Immunology·P MarchettiG Kroemer
Jan 1, 1997·Annual Review of Immunology·H NakamuraJ Yodoi
Nov 14, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B T OlejnickaU T Brunk
Apr 29, 1998·Annual Review of Physiology·G KroemerM Resche-Rigon
Jun 13, 1998·The Journal of Biological Chemistry·J Cai, D P Jones
Aug 28, 1998·Science·G Evan, T Littlewood
Feb 20, 1999·Cell·U JakobJ C Bardwell
Apr 27, 1999·The Journal of Pharmacy and Pharmacology·H Cable, J B Lloyd
Aug 24, 1999·The Journal of Biological Chemistry·Q A SunV N Gladyshev
Mar 14, 2000·Redox Report : Communications in Free Radical Research·U T Brunk, I Svensson
Jun 20, 2000·FEBS Letters·F Antunes, E Cadenas
Nov 1, 1964·Biochemistry·K A CALDWELL, A L TAPPEL

❮ Previous
Next ❯

Citations

May 31, 2007·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Virgínia Cielo RechClovis Milton Duval Wannmacher
Jan 26, 2008·In Vitro Cellular & Developmental Biology. Animal·David E StevensonJingli Zhang
Mar 20, 2004·Journal of Hepatology·Ursula RauenHerbert de Groot
Mar 31, 2004·Molecular Aspects of Medicine·Enrique Cadenas
Oct 24, 2002·Neurobiology of Aging·Hagen SchroeterCatherine Rice-Evans
Oct 11, 2002·Free Radical Biology & Medicine·Felice D'Agnillo, Abdu I Alayash
Apr 21, 2009·Chemical Reviews·Thomas W MillerDavid D Roberts
Apr 7, 2007·Journal of the American Chemical Society·Jason N LaButtiKent S Gates
Apr 9, 2008·Chemical Research in Toxicology·Shahrzad Tafazoli, Peter J O'Brien
Jun 16, 2007·Cell Death and Differentiation·A P KumarM-V Clément
Jul 26, 2003·Antioxidants & Redox Signaling·Alberto BoverisAna Navarro
Nov 1, 2003·Antioxidants & Redox Signaling·Alberto BoverisLidia E Costa
May 9, 2006·Antioxidants & Redox Signaling·James R Stone, Suping Yang
Nov 23, 2006·Antioxidants & Redox Signaling·Marat V AvshalumovMargaret E Rice
Jun 6, 2009·Antioxidants & Redox Signaling·Virgínia Oliveira-MarquesFernando Antunes
Jun 28, 2011·Antioxidants & Redox Signaling·Malcolm J Jackson
Apr 26, 2012·Antioxidants & Redox Signaling·Fei YinEnrique Cadenas
Jun 19, 2008·Molecular and Cellular Biology·Amina A Qutub, Aleksander S Popel
Mar 21, 2007·Biochemistry. Biokhimii︠a︡·O N Oktyabrsky, G V Smirnova
Sep 29, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Daniela TakeshitaSang Won Han
May 9, 2008·American Journal of Physiology. Cell Physiology·Behnam SaberiDerick Han
Jun 15, 2011·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Margaret E Rice
Aug 24, 2013·Biological & Pharmaceutical Bulletin·Xingyu ZhaoZhengli Luo
Jun 12, 2008·PloS One·Soledad GalliJuan José Poderoso
May 30, 2002·Biological Chemistry·Clinton S Boyd, Enrique Cadenas
Jul 19, 2011·Journal of Clinical Biochemistry and Nutrition·Shuyu PiaoChaekyun Kim
Apr 11, 2012·International Journal of Molecular Sciences·Marijke JozefczakAnn Cuypers
Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sunny ParkJames A Imlay
Jan 3, 2014·Antioxidants & Redox Signaling·Julie A ReiszCristina M Furdui
Feb 11, 2014·Trends in Molecular Medicine·Nuno Raimundo
May 3, 2011·Cellular and Molecular Life Sciences : CMLS·Wolfhardt FreinbichlerLaura Della Corte
May 15, 2015·Biomolecules·Nikolaus Bresgen, Peter M Eckl
Jan 28, 2012·Free Radical Biology & Medicine·Rosa Bretón-RomeroSantiago Lamas
Oct 4, 2011·Free Radical Biology & Medicine·Margaret E TomeMargaret M Briehl

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis