PMID: 9557644Apr 29, 1998Paper

Cellular transcription factors enhance herpes simplex virus type 1 oriS-dependent DNA replication

Journal of Virology
A T Nguyen-Huynh, P A Schaffer

Abstract

The herpes simplex virus type 1 (HSV-1) origin of DNA replication, oriS, contains three binding sites for the viral origin binding protein (OBP) flanked by transcriptional regulatory elements of the immediate-early genes encoding ICP4 and ICP22/47. To assess the role of flanking sequences in oriS function, plasmids containing oriS and either wild-type or mutant flanking sequences were tested in transient DNA replication assays. Although the ICP4 and ICP22/47 regulatory regions were shown to enhance oriS function, most individual elements in these regions, including the VP16-responsive TAATGARAT elements, were found to be dispensable for oriS function. In contrast, two oriS core-adjacent regulatory (Oscar) elements, OscarL and OscarR, at the base of the oriS palindrome were shown to enhance oriS function significantly and additively. Specifically, mutational disruption of either element reduced oriS-dependent DNA replication by 60 to 70%, and disruption of both elements reduced replication by 90%. The properties of protein-DNA complexes formed in gel mobility shift assays using uninfected and HSV-1-infected Vero cell nuclear extracts demonstrated that both OscarL and OscarR are binding sites for cellular proteins. Whereas OscarR...Continue Reading

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Citations

Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mauricio L NogueiraThomas M Kristie
May 16, 2007·Cell Research·Gautam R BedadalaShao-Chung V Hsia
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Alexander M MakhovJack D Griffith
Oct 2, 2009·The Journal of General Virology·Evgeny A GlazovTimothy J Mahony
Jun 10, 2008·Nature Structural & Molecular Biology·Julia Romero, Hoyun Lee
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·A JacobsC Fraefel

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