Cellular transcripts of chicken brain tissues in response to H5N1 and Newcastle disease virus infection.

Virology Journal
Vinod R M T BalasubramaniamSharifah S Hassan

Abstract

Highly-pathogenic avian influenza (HPAI) H5N1 and Newcastle disease (ND) viruses are the two most important poultry viruses in the world, with the ability to cause classic central nervous system dysfunction in poultry and migratory birds. To elucidate the mechanisms of neurovirulence caused by these viruses, a preliminary study was design to analyze host's cellular responses during infections of these viruses. An improved mRNA differential display technique (Gene Fishing™) was undertaken to analyze differentially expressed transcripts regulated during HPAI H5N1 and velogenic neurotropic NDV infections of whole brain of chickens. The identification of differentially expressed genes (DEGs) was made possible as this technique uses annealing control primers that generate reproducible, authentic and long PCR products that are detectable on agarose gels. Twenty-three genes were identified to be significantly regulated during infections with both viruses, where ten of the genes have been selected for validation using a TaqMan® based real time quantitative PCR assay. Some of the identified genes demonstrated to be key factors involving the cytoskeletal system, neural signal transduction and protein folding during stress. Interestingly,...Continue Reading

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Citations

Oct 28, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Emma E DavenportJulian C Knight
Apr 26, 2018·International Journal of Molecular Sciences·Yan-Qing JiaZeng-Qi Yang
Nov 7, 2017·Animal : an International Journal of Animal Bioscience·W Drobik-CzwarnoJ C M Dekkers
Apr 4, 2021·Pathogens·Thomas HenziLuis Filgueira

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Datasets Mentioned

BETA
HM172312

Methods Mentioned

BETA
PCR
protein folding

Software Mentioned

BLASTn
Primer3
BLAST
Basic Local Alignment Search Tool ( BLAST )

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