Cellular uptake and photodynamic activity of protein nanocages containing methylene blue photosensitizing drug

Photochemistry and Photobiology
Fei YanTuan Vo-Dinh

Abstract

This study reports that photosensitizers encapsulated in supramolecular protein cages can be internalized by tumor cells and can deliver singlet oxygen intracellularly for photodynamic therapy (PDT). As an alternative to other polymeric and/or inorganic nanocarriers and nanoconjugates, which may also deliver photosensitizers to the inside of the target cells, protein nanocages provide a unique vehicle of biological origin for the intracellular delivery of photosensitizing molecules for PDT by protecting the photosensitizers from reactive biomolecules in the cell membranes, and yet providing a coherent, critical mass of destructive power (by way of singlet oxygen) upon specific light irradiation for photodynamic therapy of tumor cells. As a model, we demonstrated the successful encapsulation of methylene blue (MB) in apoferritin via a dissociation-reassembly process controlled by pH. The resulting MB-containing apoferritin nanocages show a positive effect on singlet oxygen production, and cytotoxic effects on MCF-7 human breast adenocarcinoma cells when irradiated at the appropriate wavelength (i.e. 633 nm).

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jun 1, 1973·The Biochemical Journal·R R Crichton, C F Bryce
Nov 1, 1996·Photochemistry and Photobiology·J G Levy, M Obochi
Nov 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·G Jori
Jun 24, 1998·Journal of the National Cancer Institute·T J DoughertyQ Peng
Mar 19, 2002·Journal of Photochemistry and Photobiology. B, Biology·Yvette Niamien KonanEric Allémann
Dec 15, 2004·Photochemistry and Photobiology·Wei TangMartin A Philbert
Aug 4, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Martina E WiederDavid A Russell
Oct 22, 2008·Advanced Drug Delivery Reviews·Dev Kumar ChatterjeeYong Zhang
May 14, 2009·Chemical Communications : Chem Comm·Xavier RagàsSanti Nonell

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Citations

Apr 5, 2014·Chemistry of Materials : a Publication of the American Chemical Society·Hyung Ki YoonRaoul Kopelman
Sep 3, 2011·Photochemistry and Photobiology·Bonnie I Kruft, Alexander Greer
Jan 5, 2011·Microbial Cell Factories·Esther Vázquez, Antonio Villaverde
Nov 19, 2014·Nanomedicine·Zbynek HegerRene Kizek
Oct 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Minmin LiangXiyun Yan
Dec 10, 2015·Nanomedicine·Amar Bt Ghisaidoobe, Sang J Chung
Mar 17, 2016·Critical Reviews in Food Science and Nutrition·Jiachen ZangFazheng Ren
Mar 12, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alyssa MasterAnirban Sen Gupta
Feb 11, 2012·Current Opinion in Biotechnology·Scott T Retterer, Michael L Simpson
Dec 26, 2016·Protein Expression and Purification·Jun GuoJiang-Wei Li
Jun 13, 2017·Nanomaterials·Kyong-Hoon ChoiBong Joo Park
Oct 7, 2016·Chemical Society Reviews·Yizhong ShenHong-Yuan Chen
Jul 28, 2018·Protein Science : a Publication of the Protein Society·Katherine W PulsipherIvan J Dmochowski
Nov 7, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Krzysztof SztanderaBarbara Klajnert-Maculewicz
Jul 31, 2013·Journal of Biophotonics·Timea SimonSimion Astilean
May 16, 2019·Analytical and Bioanalytical Chemistry·Xiaoqiang WangChao Wang
Sep 9, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jiuyang HeXiyun Yan
Feb 13, 2021·International Journal of Molecular Sciences·Katarzyna KurzątkowskaMaria Hepel
Dec 22, 2011·Journal of the American Chemical Society·Frédéric SchmittBruno Therrien
Feb 17, 2015·Chemical Reviews·Günther JutzAlexander Böker

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