PMID: 8584749Oct 20, 1995Paper

Cellular uptake of intracerebrally administered oligodeoxynucleotides in mouse brain

Regulatory Peptides
S OgawaD W Pfaff

Abstract

Intracerebral diffusion, cellular uptake and intracellular localization of oligodeoxynucleotides (ODN) after their microinjection in mouse brains were examined. Using either tetramethylrhodamine-5-(and -6)-isothiocyanate (TRITC)- or gamma-33P ATP-labeled ODNs, it was found that both phosphodiester ODNs (D-ODN) and phosphorothioate ODNs (S-ODN) quickly diffused (up to about 500 microns) and were taken up by many cells around the injection site as early as 15 min after administration. Fluorescence labeling intensity and silver grain accumulation of D-ODNs were greatly reduced by 4 h after injection, whereas those of S-ODNs were stable beyond at least 8 h after injection. Most of labeled ODNs were found in neuronal cells as identified by immunocytochemistry for neurofilament, NF 200, and to a much lesser extent in astrocytic cells as identified by immunocytochemistry for glial fibrillary acidic protein.

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Citations

May 10, 2003·Brain Research. Brain Research Reviews·Dirk Van OekelenJosée E Leysen
Feb 21, 2002·Progress in Neuro-psychopharmacology & Biological Psychiatry·Alon ShamirGalila Agam
Jun 21, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mohan Babu Appaiahgari, Sudhanshu Vrati
Jan 20, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christina H LiuPhilip K Liu
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J F Guzowski, J L McGaugh
Mar 24, 2015·Advanced Drug Delivery Reviews·Melvin M EversWilleke M C van Roon-Mom
Oct 19, 2016·Nucleic Acid Therapeutics·João Casaca-CarreiraAli Jahanshahi
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Nov 19, 2019·Brain : a Journal of Neurology·Ana C SilvaLuis Pereira de Almeida
Apr 13, 2000·Cancer Control : Journal of the Moffitt Cancer Center·H H Engelhard
Jul 14, 2020·Medicinal Research Reviews·Epaminondas Doxakis
Jul 3, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Mengsi LinJiaying Qiu

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