PMID: 8458833Apr 1, 1993Paper

Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase

Journal of Bacteriology
A MeinkeR A Warren

Abstract

Five cellulose-binding polypeptides were detected in Cellulomonas fimi culture supernatants. Two of them are CenA and CenB, endo-beta-1,4-glucanases which have been characterized previously; the other three were previously uncharacterized polypeptides with apparent molecular masses of 120, 95, and 75 kDa. The 75-kDa cellulose-binding protein was designated endoglucanase D (CenD). The cenD gene was cloned and sequenced. It encodes a polypeptide of 747 amino acids. Mature CenD is 708 amino acids long and has a predicted molecular mass of 74,982 Da. Analysis of the predicted amino acid sequence of CenD shows that the enzyme comprises four domains which are separated by short linker polypeptides: an N-terminal catalytic domain of 405 amino acids, two repeated sequences of 95 amino acids each, and a C-terminal domain of 105 amino acids which is > 50% identical to the sequences of cellulose-binding domains in Cex, CenA, and CenB from C. fimi. Amino acid sequence comparison placed the catalytic domain of CenD in family A, subtype 1, of beta-1,4-glycanases. The repeated sequences are more than 40% identical to the sequences of three repeats in CenB and are related to the repeats of fibronectin type III. CenD hydrolyzed the beta-1,4-glu...Continue Reading

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Citations

Dec 1, 1994·Journal of Molecular Evolution·E LittleR F Doolittle
Sep 20, 1997·The Journal of Biological Chemistry·E J JervisD G Kilburn
Jan 7, 2006·Chembiochem : a European Journal of Chemical Biology·Spencer J WilliamsStephen G Withers
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Oct 24, 2007·Chembiochem : a European Journal of Chemical Biology·Omid HekmatStephen G Withers
Dec 22, 2007·Annals of the New York Academy of Sciences·Roy H Doi
Jul 8, 2018·Biotechnology Journal·Musashi TakenakaAkihiko Kondo
Mar 2, 2002·Applied and Environmental Microbiology·Donald Y KobayashiPeter V Oudemans
Jan 25, 2021·Biotechnology for Biofuels·James LiHarry Brumer
Sep 3, 1999·Applied and Environmental Microbiology·A E CazemierH J Op den Camp
May 29, 1999·Applied and Environmental Microbiology·D StollR A Warren
Apr 3, 2001·Protein Expression and Purification·A B BorastonD G Kilburn
Dec 19, 2000·Biochemical and Biophysical Research Communications·J O KimH D Yun
Jan 1, 1997·Biotechnology Advances·P ChaudharyD N Deobagkar

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