Cellulose degradation in Gastrophysa viridula (Coleoptera: Chrysomelidae): functional characterization of two CAZymes belonging to glycoside hydrolase family 45 reveals a novel enzymatic activity

Insect Molecular Biology
André BuschYannick Pauchet

Abstract

Cellulose is a major component of the primary and secondary cell walls in plants. Cellulose is considered to be the most abundant biopolymer on Earth and represents a large potential source of metabolic energy. Yet, cellulose degradation is rare and mostly restricted to cellulolytic microorganisms. Recently, various metazoans, including leaf beetles, have been found to encode their own cellulases, giving them the ability to degrade cellulose independently of cellulolytic symbionts. Here, we analyzed the cellulosic capacity of the leaf beetle Gastrophysa viridula, which typically feeds on Rumex plants. We identified three putative cellulases member of two glycoside hydrolase (GH) families, namely GH45 and GH9. Using heterologous expression and functional assays, we demonstrated that both GH45 proteins are active enzymes, in contrast to the GH9 protein. One GH45 protein acted on amorphous cellulose as an endo-β-1,4-glucanase, whereas the other evolved to become an endo-β-1,4-xyloglucanase. We successfully knocked down the expression of both GH45 genes using RNAi, but no changes in weight gain or mortality were observed compared to control insects. Our data indicated that the breakdown of these polysaccharides in G. viridula may f...Continue Reading

References

Jul 1, 1988·Journal of Bacteriology·M McGavin, C W Forsberg
Oct 23, 1997·Journal of Biotechnology·M Schülein
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·G SmantJ Bakker
Aug 5, 1998·Nature·H WatanabeN Lo
Nov 18, 1998·Current Opinion in Structural Biology·E A BayerY Shoham
Oct 9, 1999·Biotechnology Progress·S D MansfieldJ N Saddler
Dec 28, 1999·Insect Biochemistry and Molecular Biology·C Girard, L Jouanin
May 6, 2003·Bioscience, Biotechnology, and Biochemistry·Toshiaki TanabeMasaru Mitsutomi
Sep 15, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Seong Jin LeeByung Rae Jin
Mar 15, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Seong Jin LeeByung Rae Jin
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Oct 24, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Rui GuoFu-Kun Zhao
Apr 22, 2008·Current Opinion in Plant Biology·J Paul Knox
Jun 3, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Namjung KimByung Rae Jin
Mar 7, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kentaro Sakamoto, Haruhiko Toyohara
Sep 17, 2009·Annual Review of Entomology·Hirofumi Watanabe, Gaku Tokuda
Aug 17, 2010·Applied and Environmental Microbiology·Machiko TakahashiTakumi Takeda
Oct 6, 2010·Plant Physiology·Kenneth Keegstra
Dec 4, 2010·Journal of Insect Physiology·Jonathan D WillisJuan L Jurat-Fuentes
Dec 24, 2010·PloS One·Yannick PauchetRichard H Ffrench-Constant
May 24, 2012·Insect Biochemistry and Molecular Biology·Chia-Jung ChangYu-Chan Chao
Jul 17, 2012·Plant Science : an International Journal of Experimental Plant Biology·Katia RuelJean-Paul Joseleau
Oct 12, 2012·The Journal of Biological Chemistry·Leonid O SukharnikovIgor B Zhulin
Nov 24, 2012·Plant Physiology·Lynne H ThomasMichael C Jarvis
Jun 27, 2013·Planta·Markus PaulyGuangyan Xiong
Nov 13, 2013·Journal of Insect Physiology·Matan ShelomiGaku Arakawa
Apr 1, 1996·Journal of Chemical Ecology·A M RossiD R Strong
Nov 26, 2013·Nucleic Acids Research·Vincent LombardBernard Henrissat
Dec 1, 2014·Journal of Insect Science·Arnubio Valencia JiménezBlair D Siegfried
Dec 3, 2014·Current Opinion in Plant Biology·Daniel J Cosgrove
Feb 9, 2016·Insect Biochemistry and Molecular Biology·Matan ShelomiYannick Pauchet
Jan 1, 2014·Plants·Alex SchultinkMarkus Pauly

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.