CENP-T proteins are conserved centromere receptors of the Ndc80 complex.

Nature Cell Biology
Alexander SchleifferStefan Westermann

Abstract

Centromeres direct the assembly of kinetochores, microtubule-attachment sites that allow chromosome segregation on the mitotic spindle. Fundamental differences in size and organization between evolutionarily distant eukaryotic centromeres have in many cases obscured general principles of their function. Here we demonstrate that centromere-binding proteins are highly conserved between budding yeast and humans. We identify the histone-fold protein Cnn1(CENP-T) as a direct centromere receptor of the microtubule-binding Ndc80 complex. The amino terminus of Cnn1 contains a conserved peptide motif that mediates stoichiometric binding to the Spc24-25 domain of the Ndc80 complex. Consistent with the critical role of this interaction, artificial tethering of the Ndc80 complex through Cnn1 allows mini-chromosomes to segregate in the absence of a natural centromere. Our results reveal the molecular function of CENP-T proteins and demonstrate how the Ndc80 complex is anchored to centromeres in a manner that couples chromosome movement to spindle dynamics.

References

May 24, 1991·Science·A LupasJ Stock
Jan 1, 1996·Methods in Enzymology·J C Wootton, S Federhen
Feb 1, 1997·Current Opinion in Structural Biology·S K BurleyF Shu
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Jun 20, 2003·Current Biology : CB·Avital A RodalDavid G Drubin
Oct 29, 2003·The Journal of Cell Biology·Stefan WestermannGeorjana Barnes
Nov 25, 2003·Genes & Development·Peter De WulfPeter K Sorger
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Aug 5, 2005·The EMBO Journal·Xingkun LiuXiangwei He
Mar 28, 2006·Genome Biology·Patrick MeraldiPeter K Sorger
Apr 20, 2006·Nature Cell Biology·Daniel R FoltzDon W Cleveland
Aug 22, 2006·Current Opinion in Microbiology·Kathleen Sandman, John N Reeve
Mar 17, 2007·Annual Review of Biochemistry·Stefan WestermannGeorjana Barnes
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon
Dec 21, 2007·Molecular Biology of the Cell·Tetsuya HoriTatsuo Fukagawa
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Iain M Cheeseman, Arshad Desai
Mar 29, 2008·Briefings in Bioinformatics·Kazutaka Katoh, Hiroyuki Toh
May 9, 2008·Nucleic Acids Research·Christian ColeGeoffrey J Barton
Nov 4, 2008·Nucleic Acids Research·Ivica LetunicPeer Bork
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Jul 22, 2009·The Journal of Cell Biology·Miho AmanoTatsuo Fukagawa
Jul 25, 2009·The EMBO Journal·Stefano Santaguida, Andrea Musacchio
Aug 18, 2009·Nature Cell Biology·Soni LacefieldAndrew W Murray
Sep 22, 2009·Cell·Harmit S Malik, Steven Henikoff
Oct 6, 2009·Methods in Molecular Biology·Marco Antonio MendozaFranz Klein
Nov 19, 2009·Nucleic Acids Research·Robert D FinnAlex Bateman
Mar 4, 2010·Nature Reviews. Molecular Cell Biology·Paul B Talbert, Steven Henikoff
May 19, 2010·The Journal of Cell Biology·Fabienne LampertStefan Westermann
Jun 23, 2010·The Journal of Cell Biology·Christopher W CarrollAaron F Straight
Aug 20, 2010·BMC Bioinformatics·L Steven JohnsonElon Portugaly
Sep 8, 2010·The Journal of Cell Biology·Daniel P MaskellMartin R Singleton
Sep 8, 2010·The Journal of Cell Biology·Arsen PetrovicAndrea Musacchio

❮ Previous
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Citations

Mar 14, 2013·Trends in Cell Biology·Stefan Westermann, Alexander Schleiffer
Apr 20, 2013·Trends in Biochemical Sciences·Luying JiaHongtao Yu
May 9, 2012·Nature Cell Biology·Lucy J BockPeter De Wulf
May 9, 2012·Nature Cell Biology·Karen E Gascoigne, Iain M Cheeseman
Sep 22, 2012·Journal of Biochemistry·Kozo Tanaka
May 3, 2013·Open Biology·Bungo Akiyoshi, Keith Gull
Sep 13, 2013·Open Biology·Sonali BhattacharjeeMatthew C Whitby
Feb 19, 2013·Journal of Cell Science·Dileep Varma, E D Salmon
Oct 3, 2012·PLoS Genetics·Mickaël Durand-DubiefPatrick Varga-Weisz
Feb 14, 2014·Open Biology·Carsten DornblutKevin F Sullivan
Dec 7, 2013·Chromosoma·Gina V Caldas, Jennifer G DeLuca
May 3, 2014·Open Biology·Lakxmi SubramanianRobin C Allshire
Jul 10, 2014·ELife·Federica BasilicoAndrea Musacchio
Jun 27, 2014·Chromosoma·Francesca Malvezzi, Stefan Westermann
May 20, 2014·Cell Biochemistry and Biophysics·C George Priya DossS Debottam
Oct 8, 2014·The Journal of Cell Biology·Dan LuDavid O Morgan
Feb 22, 2013·Molecular Biology of the Cell·Ngang Heok TangTakashi Toda
Aug 20, 2014·The Journal of Cell Biology·Peter HornungStefan Westermann
Jun 26, 2012·Cellular and Molecular Life Sciences : CMLS·Madison E StellfoxDaniel R Foltz
Feb 16, 2016·Trends in Cell Biology·Ines A DrinnenbergHarmit S Malik
Jul 1, 2015·The Journal of Cell Biology·Kerstin KlareAndrea Musacchio
Mar 17, 2015·International Journal of Molecular Sciences·Christian AbendrothStephan Diekmann
Oct 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Emily M KudalkarTrisha N Davis
Nov 26, 2015·Nature Reviews. Molecular Cell Biology·Kara L McKinley, Iain M Cheeseman
Dec 17, 2015·The Journal of Cell Biology·Itaru SamejimaWilliam C Earnshaw
Mar 16, 2013·Current Opinion in Cell Biology·Frederick G Westhorpe, Aaron F Straight
Nov 17, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jakob Nilsson
Jan 22, 2013·The EMBO Journal·Tatsuya NishinoTatsuo Fukagawa
Apr 4, 2016·Current Opinion in Structural Biology·Marion E PesentiAndrea Musacchio
Sep 9, 2015·The Journal of Cell Biology·David J Wynne, Hironori Funabiki
Sep 9, 2015·Nature Communications·Aussie SuzukiEdward D Salmon
Jul 15, 2015·Molecular Biology of the Cell·Régis E MeyerDean S Dawson
Mar 29, 2014·FEMS Microbiology Reviews·Yuya YamagishiYoshinori Watanabe
May 9, 2015·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Feb 18, 2014·Current Opinion in Cell Biology·Sandra Catania, Robin C Allshire
Apr 22, 2015·Current Biology : CB·Sana Afreen, Dileep Varma
Jan 15, 2015·Nucleic Acids Research·Jörg LindeOliver Kurzai
Jan 21, 2015·The Journal of Cell Biology·Soonjoung Kim, Hongtao Yu

❮ Previous
Next ❯

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