"Center punch" and "whole spot" bioanalysis of apixaban in human dried blood spot samples by UHPLC-MS/MS

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Naiyu ZhengMark E Arnold

Abstract

Apixaban (Eliquis™) was developed by Bristol-Myers Squibb (BMS) and Pfizer to use as an antithrombotic/anticoagulant agent and has been recently approved for the prevention of stroke and systemic embolism in patients with nonvalvular atrial fibrillation. A clinical study of apixaban, sponsored by BMS and Pfizer, included a pilot exploratory portion to evaluate the potential for future drug concentration monitoring using dried blood spot (DBS) sample collection. For DBS sample collection, a fixed blood volume was dispensed onto a DBS card by either regular volumetric pipette (venous blood collection) or capillary dispenser (finger prick blood collection). A 96-well semi-automated liquid-liquid extraction sample preparation procedure was developed to provide clean extracts for UHPLC-MS/MS quantitation. Assays using both partial-spot center punch and whole spot punch were developed and validated. The linear dynamic ranges for all the analyses were from 0.5 to 500 ng/mL. The coefficient of determination (r(2)) values was >0.9944 for all the validation runs. For the center punch approach, the intra-assay precision (%CV) was within 4.4% and inter-assay precision was within 2.6%. The assay accuracy, expressed as %Dev., was within ± 5....Continue Reading

References

Dec 18, 2009·Biomedical Chromatography : BMC·Wenkui Li, Francis L S Tse
Dec 22, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Joseph M LuettgenAlan R Rendina
Jan 19, 2012·Current Drug Targets·Ritesh AgrawalS N Dikshit

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Citations

Sep 5, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Long YuanAnne-Françoise Aubry
Apr 27, 2016·Journal of Thrombosis and Thrombolysis·Wan-Li ZhangHuan-Jie Huang
May 24, 2016·Bioanalysis·Shirish YakkundiJames McElnay
Oct 27, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Artem D RogachevNariman F Salakhutdinov
Feb 21, 2018·The Analyst·H Kočová VlčkováL Nováková
Aug 28, 2021·Drug Testing and Analysis·Alina A OkhinaNariman F Salakhutdinov
Sep 3, 2021·Bioanalysis·Vera de KleijneMariëtte T Ackermans

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