Central and Peripheral Nervous System Progenitors Derived from Human Pluripotent Stem Cells Reveal a Unique Temporal and Cell-Type Specific Expression of PMCAs

Frontiers in Cell and Developmental Biology
Muwan ChenMark Denham

Abstract

The P-type ATPases family consists of ion and lipid transporters. Their unique diversity in function and expression is critical for normal development. In this study we investigated human pluripotent stem cells (hPSC) and different neural progenitor states to characterize the expression of the plasma membrane calcium ATPases (PMCAs) during human neural development and in mature mesencephalic dopaminergic (mesDA) neurons. Our RNA sequencing data identified a dynamic change in ATPase expression correlating with the differentiation time of the neural progenitors, which was independent of the neuronal progenitor type. Expression of ATP2B1 and ATP2B4 were the most abundantly expressed, in accordance with their main role in Ca2+regulation and we observed all of the PMCAs to have a subcellular punctate localization. Interestingly in hPSCs ATP2B1 and ATP2B3 were highly expressed in a cell cycle specific manner and ATP2B2 and ATP2B4 were highly expressed in a hPSC sub-population. In neural rosettes a strong apical PMCA expression was identified in the luminal region. Lastly, we confirmed all PMCAs to be expressed in mesDA neurons, however at varying levels. Our results reveal that PMCA expression dynamically changes during stem cell dif...Continue Reading

References

Jan 1, 1995·Neuron·L Stehno-BittelD E Clapham
Feb 14, 1997·Brain Research·T P StaufferE Carafoli
Sep 24, 1998·The Journal of Membrane Biology·J T Penniston, A Enyedi
Jan 12, 2001·Physiological Reviews·E E Strehler, D A Zacharias
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·M F LeiteM H Nathanson
Jun 15, 2004·Cell Calcium·Eri YanagidaSeiko Kawano
Jan 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·R FicarellaE Carafoli
Jan 13, 2009·Biochimica Et Biophysica Acta·Karen W LeeCatherine Leclerc
Sep 18, 2009·Developmental Cell·Elena TzouanacouJean-François Nicolas
Oct 24, 2009·FEBS Letters·Tobias MaierLuis Serrano
Jun 25, 2010·The Journal of Comparative Neurology·Katharine A KenyonAlain C Burette
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Marisa Brini, Ernesto Carafoli
Sep 14, 2011·Methods in Molecular Biology·Mark Denham, Mirella Dottori
Aug 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ginevra ZanniErnesto Carafoli
Oct 10, 2012·The Journal of Cell Biology·Iman SahlyChristine Petit
Oct 23, 2012·Nature·Henning TidowPoul Nissen
Jul 9, 2013·Biochimica Et Biophysica Acta·Géza AntalffyRita Padányi
Jan 21, 2014·Cellular and Molecular Life Sciences : CMLS·Marisa BriniErnesto Carafoli
Oct 23, 2015·Cell Reports·Sarah Abdul-WajidWilliam C Smith
Feb 9, 2017·Development·Tüzer KalkanAustin Smith
Aug 1, 1991·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Danica ZivkovicRené Dohmen

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Methods Mentioned

BETA
PCR
RNA-seq
RNAseq

Software Mentioned

GraphPad Prism
ZEN
TMAP
R studio
R stats package
TopHat

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