Central memory CD4+ T cells dominate the normal cerebrospinal fluid

Cytometry. Part B, Clinical Cytometry
Marieke de GraafJan Willem Gratama

Abstract

To use cerebrospinal fluid (CSF) immune phenotyping as a diagnostic and research tool, we have set out to establish reference values of white blood cell (WBC) subsets in CSF. We assessed the absolute numbers and percentages of WBC subsets by 6-color flow cytometry in paired CSF and blood samples of 84 individuals without neurological disease who underwent spinal anaesthesia for surgery. Leukocyte (i.e., lymphocytes, granulocytes, and monocytes), lymphocyte (i.e., T [CD4(+) and CD8(+) ], NK, NKT and B cells), T cell (i.e., naïve, central memory, effector memory, and regulatory) and dendritic cell subsets (i.e., myeloid and plasmacytoid) were studied. CSF showed a predominance of T cells, while granulocytes, B and NK cells were relatively rare compared to blood. The majority of T cells in CSF consisted of CD4(+) T cells (∼70%), most of them (∼90%) with a central memory phenotype, while B cells were almost absent (<1%). Among the small population of dendritic cells in CSF, those of the myeloid subtype were more frequent than plasmacytoid dendritic cells (medians: 1.7% and 0.4% of leukocytes, respectively), whilst both subsets made up 0.2% of leukocytes in blood. This study reports reference values of absolute numbers and percentag...Continue Reading

References

Dec 1, 1995·Journal of Neuroimmunology·A SvenningssonS Stemme
Mar 16, 2000·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·H NakamuraH Kato
Feb 27, 2001·Brain : a Journal of Neurology·M PashenkovH Link
May 3, 2002·Clinical and Experimental Immunology·M PashenkovH Link
Jun 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Pia KivisäkkRichard M Ransohoff
Jul 5, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Kathleen Jentsch-UllrichAstrid Franke
Jul 26, 2005·Trends in Immunology·Britta Engelhardt, Richard M Ransohoff
Jan 30, 2008·Cytometry. Part B, Clinical Cytometry·Maurice R G O'Gorman, Lynn S Zijenah
Mar 25, 2008·Journal of Neuroimmunology·Marieke de GraafJan Willem Gratama
Sep 5, 2008·Current Protocols in Cytometry·Jaco KraanWyndham Wilson
Oct 7, 2008·Journal of Immunological Methods·Silvia Della BellaMaria Luisa Villa
Oct 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tae Kon KimKrishna V Komanduri

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Citations

Mar 15, 2011·Journal of Neurology·Marieke T de GraafPeter A E Sillevis Smitt
Apr 10, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Rhoanne C McPherson, Stephen M Anderton
Jan 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kuti BaruchMichal Schwartz
Jul 8, 2011·Clinical Microbiology Reviews·Barry B Mook-KanamoriDiederik van de Beek
Apr 27, 2012·Journal of Neuroinflammation·Shahani Noor, Emma H Wilson
Jul 25, 2013·Acta Neuropathologica·Joost SmoldersJörg Hamann
Nov 5, 2015·Neurotoxicology·Matthias LuzH Christian Fibiger
Feb 20, 2013·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·R A GroveO Graff
Jun 18, 2011·Developmental Medicine and Child Neurology·Michael R Pranzatelli
Jun 22, 2013·Cytometry. Part B, Clinical Cytometry·R Clive Landis
Feb 13, 2013·Cytometry. Part B, Clinical Cytometry·Adriaan H C de JongsteJan W Gratama
Mar 31, 2015·Brain Research·Luokun Xie, Shao-Hua Yang
Apr 20, 2013·Brain, Behavior, and Immunity·Kuti Baruch, Michal Schwartz
Aug 21, 2012·Cell Adhesion & Migration·Rick B MeekerLola C Hudson
Jun 18, 2016·Mediators of Inflammation·Tobias DahmTobias Tenenbaum
Mar 14, 2016·Cellular and Molecular Life Sciences : CMLS·Felix LuessiEsther Witsch
Oct 21, 2016·Arquivos de neuro-psiquiatria·Sérgio Monteiro de AlmeidaGisele M B Singer
Jan 18, 2018·Journal of Neuropathology and Experimental Neurology·Maxime De LaereNathalie Cools
May 14, 2011·Cytometry. Part B, Clinical Cytometry·Marieke T de GraafJan W Gratama
Jan 26, 2018·Acta Neuropathologica·Jean-François Ghersi-EgeaBritta Engelhardt
Oct 1, 2019·The Journal of Clinical Investigation·Eduardo BeltránKlaus Dornmair
Sep 11, 2019·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Masashi SakuraiMasahiro Morimoto
Oct 27, 2015·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·R Lee Mosley
Dec 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yair FisherAlon Monsonego
Feb 11, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sungpil HanBibiana Bielekova
Jun 6, 2018·Frontiers in Immunology·Itai StromingerAlon Monsonego
Jun 28, 2019·Frontiers in Immunology·Tobias V LanzLucas Schirmer
Jun 19, 2017·Acta Neuropathologica·Gijsbert P van NieropGeorges M G M Verjans
Jun 2, 2020·Expert Review of Neurotherapeutics·Joost SmoldersInge Huitinga
Aug 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Akshaya RameshMichael R Wilson
Nov 22, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jonathan RoydsConnail McCrory

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