Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection

The Journal of Immunology : Official Journal of the American Association of Immunologists
Gilson Costa MacedoSergio C Oliveira

Abstract

Brucella abortus is a facultative intracellular bacterium that infects humans and domestic animals. The enhanced susceptibility to virulent B. abortus observed in MyD88 knockout (KO) mice led us to investigate the mechanisms involved in MyD88-dependent immune responses. First, we defined the role of MyD88 in dendritic cell (DC) maturation. In vitro as well as in vivo, B. abortus-exposed MyD88 KO DCs displayed a significant impairment on maturation as observed by expression of CD40, CD86, and MHC class II on CD11c+ cells. In addition, IL-12 and TNF-alpha production was totally abrogated in MyD88 KO DCs and macrophages. Furthermore, B. abortus-induced IL-12 production was found to be dependent on TLR2 in DC, but independent on TLR2 and TLR4 in macrophages. Additionally, we investigated the role of exogenous IL-12 and TNF-alpha administration on MyD88 KO control of B. abortus infection. Importantly, IL-12, but not TNF-alpha, was able to partially rescue host susceptibility in MyD88 KO-infected animals. Furthermore, we demonstrated the role played by TLR9 during virulent B. abortus infection. TLR9 KO-infected mice showed 1 log Brucella CFU higher than wild-type mice. Macrophages and DC from TLR9 KO mice showed reduced IL-12 and una...Continue Reading

References

Apr 1, 1998·Nature·J Banchereau, R M Steinman
Aug 6, 1999·Immunity·T KawaiS Akira
Apr 5, 2001·Trends in Immunology·T Kaisho, S Akira
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L Y HuangD E Scott
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jinkyung KoGary A Splitter
Feb 28, 2002·Annual Review of Immunology·Pierre GuermonprezSebastian Amigorena
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Feb 21, 2003·European Journal of Immunology·Shivanthi P ManickasinghamCaetano Reis e Sousa
Jul 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Li-Yun HuangBasil Golding
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Aug 24, 2004·Nature Immunology·Salomé LeibundGut-LandmannWalter Reith
Sep 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guillermo H GiambartolomeiMario T Philipp
Sep 30, 2004·Nature Immunology·Akiko Iwasaki, Ruslan Medzhitov
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carl De TrezEric Muraille
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shuangping ShiSabine Ehrt
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Li-Yun HuangBasil Golding
Feb 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paula M BerguerFernando A Goldbaum
Feb 7, 2007·Seminars in Immunology·Gisa GeroldJuana L de Diego
Feb 20, 2007·Microbes and Infection·Anderson MiyoshiSergio C Oliveira
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melanie R PowerTong-Jun Lin

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Citations

Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaoyun ZhangGuangming Zhong
Mar 6, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marco Tulio R GomesSergio C Oliveira
Dec 5, 2013·Microbiology and Immunology·Ebrahim Eskandari-NasabMehdi Hadadi-Fishani
Jan 15, 2015·Infection and Immunity·Júlia Silveira FahelSergio Costa Oliveira
Mar 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·M Cruz MiragliaGuillermo H Giambartolomei
Jan 26, 2017·Frontiers in Cellular and Infection Microbiology·Maykel A AriasJulián Pardo
Sep 11, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Matsayapan PudlaPongsak Utaisincharoen
Dec 6, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Miriam M Costa FrancoSergio C Oliveira
Mar 29, 2019·European Journal of Immunology·Priscila Carneiro CamposSergio Costa Oliveira
Feb 21, 2019·Folia Microbiologica·Eric Daniel Avila-CalderónAraceli Contreras-Rodríguez
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Woraporn SukhumavasiEric Y Denkers
Jul 8, 2009·Pakistan Journal of Biological Sciences : PJBS·Md Ariful IslamIl Lee
Jun 26, 2013·Cellular Microbiology·Ivo FabrikJiri Stulik
Dec 21, 2012·European Journal of Immunology·Horst von BernuthJean-Laurent Casanova
Nov 2, 2016·European Journal of Immunology·Tatiana N SilveiraSergio C Oliveira
Jan 11, 2017·Infection and Immunity·Carolyn A LaceyJerod A Skyberg
Feb 4, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lili ChenGuangming Zhong
Apr 21, 2010·Infection and Immunity·Manuel Rodríguez-ZapataMelchor Alvarez-Mon
Aug 10, 2011·Infection and Immunity·María P Jiménez de BagüésJulián Pardo
Aug 17, 2011·Infection and Immunity·Fernanda S OliveiraSérgio C Oliveira
Dec 29, 2011·Clinical & Developmental Immunology·Fernanda S OliveiraSergio C Oliveira
Jan 7, 2016·Annual Review of Animal Biosciences·Mariana X Byndloss, Renee M Tsolis
Jul 12, 2017·Journal of Veterinary Science·Alisha W B ReyesSuk Kim
May 22, 2019·Journal of Immunology Research·Aneesh ThakurGregers Jungersen
Jul 14, 2016·Seminars in Immunopathology·Fernanda M MarimSergio C Oliveira

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