Centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection

Biosensors & Bioelectronics
Seung Jun OhTae Seok Seo

Abstract

We present a centrifugal microfluidic device which enables multiplex foodborne pathogen identification by loop-mediated isothermal amplification (LAMP) and colorimetric detection using Eriochrome Black T (EBT). Five identical structures were designed in the centrifugal microfluidic system to perform the genetic analysis of 25 pathogen samples in a high-throughput manner. The sequential loading and aliquoting of the LAMP cocktail, the primer mixtures, and the DNA sample solutions were accomplished by the optimized zigzag-shaped microchannels and RPM control. We targeted three kinds of pathogenic bacteria (Escherichia coli O157:H7, Salmonella typhimurium and Vibrio parahaemolyticus) and detected the amplicons of the LAMP reaction by the EBT-mediated colorimetric method. For the limit-of-detection (LOD) test, we carried out the LAMP reaction on a chip with serially diluted DNA templates of E. coli O157:H7, and could observe the color change with 380 copies. The used primer sets in the LAMP reaction were specific only to the genomic DNA of E. coli O157:H7, enabling the on-chip selective, sensitive, and high-throughput pathogen identification with the naked eyes. The entire process was completed in 60min. Since the proposed microsys...Continue Reading

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Citations

Nov 27, 2016·Biosensors & Bioelectronics·Maria Chiara Giuffrida, Giuseppe Spoto
Jun 18, 2017·Journal of Microbiological Methods·Xiaoping ZhangSaijun Tang
Aug 17, 2017·Sensors·Md Eshrat E Alahi, Subhas Chandra Mukhopadhyay
Jan 10, 2018·Frontiers in Microbiology·Brett E EtchebarneMary J Hughes
Nov 7, 2017·Foodborne Pathogens and Disease·Krista CarlsonSwomitra Mohanty
Dec 21, 2018·Critical Reviews in Food Science and Nutrition·Yuting ShangXiulan Sun
Jun 30, 2019·Micromachines·Lena GorgannezhadNam-Trung Nguyen
Mar 5, 2017·Essays in Biochemistry·Amornrat AroonnualSurang Chankhamhaengdecha
Aug 29, 2020·The Analyst·Chunyu LiBo Ma
Aug 30, 2017·Micromachines·Ismail Hussain Kamal BashaNor Hisham Bin Hamid
May 24, 2016·Micromachines·Luc Bissonnette, Michel G Bergeron
Feb 6, 2016·Micromachines·Minghui TangHo-Pui Ho
Nov 21, 2020·Analytical Methods : Advancing Methods and Applications·Tianzeng HuangHongmei Cao
Jun 4, 2020·Applied Microbiology and Biotechnology·Zengjun JinWeihao Li
Nov 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Yogesh KumarPranita Jaiswal
Sep 2, 2018·Biosensors & Bioelectronics·Christopher R PhaneufChung-Yan Koh
May 23, 2021·International Journal of Food Microbiology·Alka RaniAndrew S Ball

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